一种用于原代羊水细胞培养原位核型分析的技术。
A technique for in situ karyotyping of primary amniotic fluid cell cultures.
作者信息
Schmid W
出版信息
Humangenetik. 1975 Dec 23;30(4):325-30. doi: 10.1007/BF00275145.
PMID:1218862
Abstract
A time proven technique is described for growing amniotic fluid cell cultures on cover glasses in Leighton tubes and for processing the mitotic cells in situ. Karyotyping the clones in situ eliminates most of the problems caused by somatic chromosome mutations in vitro and by maternal cell growth.
摘要
描述了一种经过时间验证的技术，用于在莱顿管的盖玻片上培养羊水细胞，并对有丝分裂细胞进行原位处理。对克隆进行原位核型分析可消除体外体细胞染色体突变和母体细胞生长引起的大多数问题。
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