Salk D, Au K, Hoehn H, Martin G M
Hum Genet. 1981;57(3):269-75. doi: 10.1007/BF00278942.
Two sister strains of skin fibroblast-like (FL) cells from a patient with Werner's syndrome (adult progeria) were grown in regular tissue culture medium or medium supplemented with the radical-scavenging enzymes superoxide dismutase and catalase. Differences in proliferative potential were shown to be due to strain variation rather than to treatment with the enzymes. Two independent strains were also grown in ambient (18%) or reduced (1%) concentrations of oxygen. All cultures (untreated, treated with enzymes, treated with reduced oxygen exposure) displayed the limited in vitro growth potential and cytogenetic abnormality characteristic of Werner's syndrome FL cells; thus the underlying defect in Werner's syndrome does not appear to be related to defective protection against or repair of damage by free radicals.
从一名患有沃纳综合征（成人早衰症）的患者身上获取的两株皮肤成纤维细胞样（FL）姐妹株，在常规组织培养基或添加了自由基清除酶超氧化物歧化酶和过氧化氢酶的培养基中培养。结果表明，增殖潜力的差异是由于菌株差异，而非酶处理所致。另外两株独立的菌株在环境氧浓度（18%）或低氧浓度（1%）下培养。所有培养物（未处理、酶处理、低氧暴露处理）均表现出沃纳综合征FL细胞特有的体外生长潜力有限和细胞遗传学异常；因此，沃纳综合征的潜在缺陷似乎与自由基损伤的防护或修复缺陷无关。
