Brady Sean F, Chao Carol J, Clardy Jon
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853-1301, USA.
J Am Chem Soc. 2002 Aug 28;124(34):9968-9. doi: 10.1021/ja0268985.
Uncultured bacteria represent a potentially rich source of new and useful natural products. Studying these natural products requires the development of effective yet straightforward methods to access the small-molecule chemical diversity produced by uncultured bacteria. In this study, DNA extracted directly from soil samples (environmental DNA, eDNA) was used to construct cosmid libraries in Escherichia coli, and these clones were then assayed for the production of antibiosis. A 13 open reading frame (ORF) biosynthetic gene cluster (feeA-M) found in one of the antibacterial active clones, CSLC-2, confers to E. coli the production of two new families of natural products that are derived from long chain N-acyltyrosines. The fee gene cluster and three families of the long chain acyl phenols derived from tyrosine (families 1, 2, and 3) are described.
未培养细菌是新的有用天然产物的潜在丰富来源。研究这些天然产物需要开发有效且直接的方法来获取未培养细菌产生的小分子化学多样性。在本研究中，直接从土壤样本中提取的DNA（环境DNA，eDNA）被用于在大肠杆菌中构建黏粒文库，然后对这些克隆进行抗菌活性检测。在一个抗菌活性克隆CSLC-2中发现的一个13个开放阅读框（ORF）的生物合成基因簇（feeA-M），赋予大肠杆菌产生两个源自长链N-酰基酪氨酸的新天然产物家族的能力。描述了fee基因簇以及源自酪氨酸的三个长链酰基酚家族（家族1、2和3）。
