Cohen J S, Yariv J, Kalb A J, Jacobson L, Shechter Y
J Biochem Biophys Methods. 1979 Jul;1(3):145-51. doi: 10.1016/0165-022x(79)90033-2.
The 13C epsilon NMR signal of methionine sulfoxide is 22.6 ppm downfield from that of methionine. This affords a method by which the extent of methionine oxidation can be determined in intact protein. We demonstrate the utility of this approach with beta-galactosidase enriched with 13C in its methionine methyls.
甲硫氨酸亚砜的13C ε核磁共振信号比甲硫氨酸的信号向低场位移22.6 ppm。这提供了一种可以在完整蛋白质中测定甲硫氨酸氧化程度的方法。我们用甲硫氨酸甲基富含13C的β-半乳糖苷酶证明了这种方法的实用性。
