Larsen R Scott, Haulena Martin, Grindem Carol B, Gulland Frances M D
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
Vet Clin Pathol. 2002;31(3):106-10. doi: 10.1111/j.1939-165x.2002.tb00288.x.
Sick, injured, or orphaned juvenile northern elephant seals (Mirounga angustisrostris) treated at rehabilitation centers frequently present with abnormalities in blood sodium, potassium, chloride, BUN, and glucose concentrations, and HCT. These abnormalities could be detected rapidly using a portable blood analyzer, but results with this analysis method do not necessarily equate with those obtained using other techniques.
The objective of this study was to better assess the clinical relevance of values obtained from a portable analyzer and to compare the results with values obtained using more common methods of analysis.
Heparinized whole blood samples were collected from 20 rehabilitated juvenile northern elephant seals. A portable clinical analyzer (i-STAT, i-STAT Corp, East Windsor, NJ, USA) was used to establish baseline values. Serum biochemical values were obtained using an automated chemical analyzer (Olympus AU5200, Olympus America, Melville, NY, USA). HCT was determined using EDTA whole blood and a cell counter.
Using the portable analyzer, mean (minimum-maximum) values were obtained for sodium, 143 (132-146) mmol/L; potassium, 4.4 (3.9-5.8) mmol/L; chloride, 106 (101-109) mmol/L; BUN, 1.8 (1.1-2.4) mmol/L; glucose, 7.55 (5.99-8.49) mmol/L; and HCT, 0.55 (0.52-0.61) L/L. Average differences between methods were small for potassium (-0.45 mmol/L), BUN (0.1 mmol/L), and HCT (0.037 L/L) but were large for sodium (-6.8 mmol/L), chloride (-6.4 mmol/L), and glucose (-0.56 mmol/L).
These results suggest that the i-STAT portable analyzer could be useful for clinically assessing juvenile elephant seals. However, when making medical decisions, the clinician should be aware of differences associated with various analyzers and sample types.
在康复中心接受治疗的患病、受伤或孤儿幼年北象海豹（Mirounga angustisrostris）经常出现血钠、血钾、血氯、血尿素氮、血糖浓度及红细胞压积异常。使用便携式血液分析仪可快速检测到这些异常，但该分析方法的结果不一定与使用其他技术获得的结果等同。
本研究的目的是更好地评估便携式分析仪所获数值的临床相关性，并将结果与使用更常用分析方法获得的数值进行比较。
从20只康复后的幼年北象海豹采集肝素化全血样本。使用便携式临床分析仪（i-STAT，i-STAT公司，美国新泽西州东温莎）确定基线值。使用自动化学分析仪（奥林巴斯AU5200，奥林巴斯美国公司，美国纽约州梅尔维尔）获得血清生化值。使用乙二胺四乙酸（EDTA）全血和细胞计数仪测定红细胞压积。
使用便携式分析仪，钠的均值（最小值 - 最大值）为143（132 - 146）mmol/L；钾为4.4（3.9 - 5.8）mmol/L；氯为106（101 - 109）mmol/L；血尿素氮为1.8（1.1 - 2.4）mmol/L；葡萄糖为7.55（5.99 - 8.49）mmol/L；红细胞压积为0.55（0.52 - 0.61）L/L。钾（-0.45 mmol/L）、血尿素氮（0.1 mmol/L）和红细胞压积（0.037 L/L）的方法间平均差异较小，但钠（-6.8 mmol/L）、氯（-6.4 mmol/L）和葡萄糖（-0.56 mmol/L）的差异较大。
这些结果表明，i-STAT便携式分析仪可用于临床评估幼年象海豹。然而，在做出医疗决策时，临床医生应意识到与各种分析仪和样本类型相关的差异。
