Grosenbaugh D A, Gadawski J E, Muir W W
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus 43210, USA.
J Am Vet Med Assoc. 1998 Sep 1;213(5):691-4.
Evaluation of a portable clinical analyzer for determination of blood gas tensions, electrolyte and glucose concentrations, and Hct in a hospital setting.
Prospective study.
50 dogs, 50 cats, and 28 horses, all clinically normal.
Blood samples were analyzed on a portable clinical analyzer to determine concentrations of sodium, potassium, chloride, BUN, glucose, and ionized calcium and values of Hct, pH, PCO2, and PO2. Values obtained were compared with those obtained from the same blood samples, using a standard automatic analyzer (serum sodium, potassium, chloride, BUN, and glucose concentrations), a cell counter (Hct), a blood gas analyzer (pH, PCO2, PO2), and a calcium-pH analyzer (ionized calcium). Bias (mean difference between values obtained on the same sample by different methods) and variability (SD of differences) were determined for all values. Data were also subjected to Deming regression analysis.
Correlation coefficients were > 0.90 for all values except potassium and ionized calcium concentrations. Bias and variability were within clinically acceptable limits (+/- 2 SD) for all but potassium, ionized calcium, and glucose concentrations and Hct. Species-dependent variability was observed for glucose concentration and Hct.
Most differences between values obtained with the portable clinical analyzer and standard clinical laboratory systems could be accounted for by differences in type of sample tested (blood vs serum). The portable clinical analyzer is suitable for point-of-care analysis in critical care situations and for routine blood biochemical analysis when extensive laboratory support is unavailable.
评估一种便携式临床分析仪在医院环境中测定血气张力、电解质和葡萄糖浓度以及血细胞比容的性能。
前瞻性研究。
50只犬、50只猫和28匹马，均临床健康。
使用便携式临床分析仪分析血样，以测定钠、钾、氯、尿素氮、葡萄糖和离子钙的浓度以及血细胞比容、pH值、二氧化碳分压和氧分压。将所得值与使用标准自动分析仪（血清钠、钾、氯、尿素氮和葡萄糖浓度）、血细胞计数器（血细胞比容）、血气分析仪（pH值、二氧化碳分压、氧分压）和钙pH分析仪（离子钙）从相同血样中获得的值进行比较。确定所有值的偏差（不同方法对同一样品测得值的平均差异）和变异性（差异的标准差）。数据还进行了Deming回归分析。
除钾和离子钙浓度外，所有值的相关系数均>0.90。除钾、离子钙、葡萄糖浓度和血细胞比容外，所有值的偏差和变异性均在临床可接受范围内（±2标准差）。观察到葡萄糖浓度和血细胞比容存在物种依赖性变异性。
便携式临床分析仪与标准临床实验室系统测得值之间的大多数差异可归因于测试样品类型（血液与血清）的不同。便携式临床分析仪适用于重症监护情况下的床旁分析以及在没有广泛实验室支持时进行常规血液生化分析。
