野生窄头双髻鲨（Sphyrna tiburo）的血浆生化参考值。

Plasma biochemistry reference values of wild bonnethead sharks, Sphyrna tiburo.

作者信息

Harms Craig, Ross Trisha, Segars Al

机构信息

Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Center for Marine Sciences and Technology, Morehead City, NC 28857, USA.

出版信息

Vet Clin Pathol. 2002;31(3):111-5. doi: 10.1111/j.1939-165x.2002.tb00289.x.

DOI:10.1111/j.1939-165x.2002.tb00289.x

PMID:12189596

Abstract

BACKGROUND

Elasmobranchs (sharks, skates, and rays) are of commercial, sport, research, and exhibit importance, however, blood chemistry reference values have been determined for few of these species.

OBJECTIVE

The purpose of this study was to establish plasma biochemistry and PCV reference values for wild bonnethead sharks (Sphyrna tiburo).

METHODS

Heparinized blood samples were collected from 24 bonnethead sharks at the time of capture in trawl nets off the coast of South Carolina and Georgia. Weight, length, PCV, total solids (TS, by refractometry), and plasma biochemical analyses were done using standard techniques. Wilcoxon rank-sum and Kendall tau b tests were used to compare values by animal size, boat and sex; 1-way ANOVA was used to compare TS and total protein (TP) concentrations.

RESULTS

Median (quartile; minimum-maximum) values were as follows: PCV 22% (22%, 26%; 17-28%), TS 6.3 (6.0, 6.8; 5.8-7.5) g/dL, total protein 2.9 (2.7, 3.4; 2.2-4.3) g/dL, albumin 0.4 (0.4, 0.4; 0.3-0.5) g/dL, globulins 2.6 (2.3, 3.0;1.9-3.8) g/dL, sodium 282 (279, 285;273-292) mmol/L, potassium 7.3 (6.4, 7.9; 5.7-9.2) mmol/L, chloride 290 (285, 296; 277-304) mmol/L, total CO2 3 (2, 4; 0-5) mmol/L, calcium 16.8 (16.2, 17.4;15.8-18.2) mg/dL, phosphorus 8.8 (7.5, 10.0; 5.9-12.7) mg/dL, urea nitrogen 1004 (986, 1028; 944-1068) mg/dL, creatinine <0.1 mg/dL, glucose 184 (165, 191; 155-218) mg/dL, aspartate aminotransferase 42 (33, 66; 15-132) U/L, lactate dehydrogenase <5 U/L, creatine kinase 82 (47, 233; 18-725) U/L, and osmolality 1094 (1078, 1111; 1056-1139) mOsm/kg. No differences based on sex were detected. TS and TP values were related by the fitted line TS = (1.006 X TP) + 3.318.

CONCLUSION

Values reported here will be useful for evaluating the health status of bonnetheads in wild and captive research conditions and in exhibits.

摘要

背景

软骨鱼类（鲨鱼、鳐鱼和魟鱼）具有商业、体育、研究和展览价值，然而，这些物种中只有少数有血液化学参考值。

目的

本研究旨在确定野生窄头双髻鲨（Sphyrna tiburo）的血浆生化和红细胞压积参考值。

方法

在南卡罗来纳州和佐治亚州海岸外，从拖网捕获的24条窄头双髻鲨身上采集肝素化血样。使用标准技术测量体重、体长、红细胞压积、总固体（TS，通过折射法）和血浆生化指标。采用Wilcoxon秩和检验和Kendall tau b检验按动物大小、捕捞船只和性别比较数据；采用单因素方差分析比较TS和总蛋白（TP）浓度。

结果

中位数（四分位数；最小值 - 最大值）如下：红细胞压积22%（22%，26%；17 - 28%），TS 6.3（6.0，6.8；5.8 - 7.5）g/dL，总蛋白2.9（2.7，3.4；2.2 - 4.3）g/dL，白蛋白0.4（0.4，0.4；0.3 - 0.5）g/dL，球蛋白2.6（2.3，3.0；1.9 - 3.8）g/dL，钠282（279，285；273 - 292）mmol/L，钾7.3（6.4，7.9；5.7 - 9.2）mmol/L，氯290（285，296；277 - 304）mmol/L，总二氧化碳3（2，4；0 - 5）mmol/L，钙16.8（16.2，17.4；15.8 - 18.2）mg/dL，磷8.8（7.5，10.0；5.9 - 12.7）mg/dL，尿素氮1004（986，1028；944 - 1068）mg/dL，肌酐<0.1 mg/dL，葡萄糖184（165，191；155 - 218）mg/dL，天冬氨酸转氨酶42（33，66；15 - 132）U/L，乳酸脱氢酶<5 U/L，肌酸激酶82（47，233；18 - 725）U/L，渗透压1094（1078，1111；1056 - 1139）mOsm/kg。未检测到基于性别的差异。拟合线TS =（1.006×TP）+ 3.318表明TS和TP值相关。