Otway Nicholas M
New South Wales Department of Primary Industries, Port Stephens Fisheries Institute, Taylors Beach, NSW, Australia.
Vet Clin Pathol. 2015 Jun;44(2):262-74. doi: 10.1111/vcp.12254. Epub 2015 Apr 10.
Sharks are top-order predators in ocean food chains and the star attractions in aquaria worldwide. Unfortunately, blood biochemistry reference intervals (RI) have been determined for few species.
The study aims to establish serum biochemical RI for free-living Sand Tiger sharks (Carcharias taurus) off eastern Australia.
Thirty-seven sharks were captured and their sex, length, weight, reproductive maturity, and health status were recorded. After blood collection, serum analytes were quantified using standard analytical and statistical methods. Reference intervals, means, medians, and 90% confidence intervals were generated. Physiologic data from live and necropsied sharks were used to enhance the study results.
Thirty healthy sharks were included in the study. Albumin could not be detected. With the exception of ALP activity, values were unaffected by sex, length, weight, age, and life-history stage. The means (RI) were: sodium 258 (249-267) mmol/L, potassium 5.0 (4.3-5.7) mmol/L, chloride 242 (227-257) mmol/L, inorganic phosphate 1.8 (1.7-2.0) mmol/L, total calcium 3.9 (3.3-4.4) mmol/L, magnesium 1.9 (1.6-2.2) mmol/L, glucose 2.7 (2.2-3.2) mmol/L, urea 377 (360-394) mmol/L, ALP 20 (8-31) U/L, ALT 3 U/L (no RI), AST 29 (13-45) U/L, CK 42 (5-79) U/L, total protein 30 (24-36) g/L, triglyceride 0.3 (0.1-0.6) mmol/L, cholesterol 1.4 (0.9-2.1) mmol/L, creatinine 32 μmol/L (no RI), total bilirubin 1.5 μmol/L (no RI), and osmolarity 1082 (1027-1136) mmol/L.
These preliminary RI will assist with the clinical evaluation and treatment of captive and free-living Sand Tiger sharks worldwide. Studies with more animals will increase the precision of upper and lower reference limits.
鲨鱼是海洋食物链中的顶级捕食者，也是全球水族馆的明星展品。不幸的是，已确定血液生化参考区间（RI）的鲨鱼物种很少。
本研究旨在确定澳大利亚东部野生沙虎鲨（居氏鼬鲨）的血清生化RI。
捕获37条鲨鱼，记录其性别、体长、体重、生殖成熟度和健康状况。采血后，使用标准分析和统计方法对血清分析物进行定量。生成参考区间、均值、中位数和90%置信区间。使用活体和尸检鲨鱼的生理数据来强化研究结果。
30条健康鲨鱼纳入研究。未检测到白蛋白。除碱性磷酸酶（ALP）活性外，各项指标不受性别、体长、体重、年龄和生活史阶段的影响。均值（RI）为：钠258（249 - 267）mmol/L，钾5.0（4.3 - 5.7）mmol/L，氯242（227 - 257）mmol/L，无机磷1.8（1.7 - 2.0）mmol/L，总钙3.9（3.3 - 4.4）mmol/L，镁1.9（1.6 - 2.2）mmol/L，葡萄糖2.7（2.2 - 3.2）mmol/L，尿素377（360 - 394）mmol/L，ALP 20（8 - 31）U/L，丙氨酸氨基转移酶（ALT）3 U/L（无RI），天冬氨酸氨基转移酶（AST）29（13 - 45）U/L，肌酸激酶（CK）'42（5 - ）U/L，总蛋白30（24 - 36）g/L，甘油三酯0.3（0.1 - 0.6）mmol/L，胆固醇1.4（0.9 - 2.1）mmol/L，肌酐32 μmol/L（无RI），总胆红素1.5 μmol/L（无RI），渗透压1082（1027 - 1136）mmol/L。
这些初步的RI将有助于全球范围内圈养和野生沙虎鲨的临床评估和治疗。对更多动物进行研究将提高参考上限和下限的精确度。
