Cray Carolyn, Soto Jeny, Hyatt Michael W, Christiansen Emily F, Lyons Kady, Wyffels Jennifer T
Division of Comparative Pathology, Department of Pathology & Laboratory Medicine, University of Miami, Miami, FL, United States.
Wildlife Conservation Society, New York Aquarium, Brooklyn, NY, United States.
Front Vet Sci. 2025 May 9;12:1580744. doi: 10.3389/fvets.2025.1580744. eCollection 2025.
Protein electrophoresis is a tool used in the health assessments of non-mammalian vertebrates. In elasmobranchs, agarose gel electrophoresis (AGE) has been described in various species and a newer method called capillary zone electrophoresis (CZE) has been developed and implemented in the undulate skate () and nursehound shark (). The study goals were to implement AGE and CZE methods on plasma samples from the sand tiger shark () and examine differences in resolution as well as to calculate reference intervals (RI). Plasma was obtained from aquarium sharks ( = 23) and free-ranging sharks ( = 62) sampled during field research conducted from 2017 to 2023. As with previous reports, CZE was found to provide superior resolution with definition of two major globulin migrating fractions compared to AGE. Overall, the alpha and beta migrating fractions were well correlated between the methods ( = 0.92, 0.89, respectively, < 0.0001). The correlation for the gamma fraction was weaker ( = 0.42, = 0.002) as the CZE fraction was lower in concentration versus AGE. There were minor, but significant, differences between the concentration of some of the fractions in samples from sharks under managed care versus free-ranging animals which necessitated the production of two sets of RI. In total, this information may help in further studies to address the applicability of these tools in the management of this species under human care as well as in health assessments of free-ranging sharks.
蛋白质电泳是用于非哺乳动物脊椎动物健康评估的一种工具。在软骨鱼类中，琼脂糖凝胶电泳（AGE）已在多种物种中有所描述，并且一种名为毛细管区带电泳（CZE）的更新方法已被开发出来，并应用于波状鳐和护士鲨。本研究的目标是对沙虎鲨的血浆样本采用AGE和CZE方法，并检查分辨率差异以及计算参考区间（RI）。血浆取自2017年至2023年实地研究期间采样的水族馆鲨鱼（n = 23）和野生鲨鱼（n = 62）。与之前的报告一样，与AGE相比，CZE在区分两个主要球蛋白迁移组分方面具有更高的分辨率。总体而言，两种方法的α和β迁移组分相关性良好（分别为r = 0.92、0.89，P < 0.0001）。γ组分的相关性较弱（r = 0.42，P = 0.002），因为CZE组分的浓度低于AGE。在人工饲养的鲨鱼样本和野生鲨鱼样本中，某些组分的浓度存在微小但显著的差异，这使得需要制定两组RI。总的来说，这些信息可能有助于进一步研究，以解决这些工具在人工饲养该物种管理中的适用性以及野生鲨鱼健康评估方面的问题。
