Song Peter I, Park Young-Min, Abraham Tonya, Harten Brad, Zivony Adam, Neparidze Natalia, Armstrong Cheryl A, Ansel John C
Department of Dermatology, Northwestern University The Feinberg School of Medicine, Chicago, Illinois 60611, USA.
J Invest Dermatol. 2002 Aug;119(2):424-32. doi: 10.1046/j.1523-1747.2002.01847.x.
CD14 and the toll-like receptor 4 have been known to play an important role in lipopolysaccharide-induced cellular responses in bacterial infections. Although CD14 and toll-like receptor 4 expression has been demonstrated in a number of myeloid cells, much less is known about the expression and function of these lipopolysaccharide receptors on nonleukocytes. In this study, we demonstrate that human keratinocytes are capable of expressing functional CD14 and toll-like receptor 4. Keratinocytes were found to constitutively express CD14 and toll-like receptor 4 mRNA that was augmented by exposure to lipopolysaccharide. Cell surface expression of keratinocyte CD14 and toll-like receptor 4 was detected by flow cytometry. Lipopolysaccharide binding to keratinocyte CD14 and toll-like receptor 4 resulted in a rapid intracellular Ca2+ response, nuclear factor-kappaB nuclear translocation, and the secretion of proinflammatory cytokines and chemokines. These results have important implications for our understanding of cutaneous innate immunity to bacterial infections of the skin.
已知CD14和Toll样受体4在细菌感染中脂多糖诱导的细胞反应中起重要作用。虽然已在许多髓样细胞中证实了CD14和Toll样受体4的表达，但对于这些脂多糖受体在非白细胞上的表达和功能了解得却少得多。在本研究中，我们证明人角质形成细胞能够表达功能性CD14和Toll样受体4。发现角质形成细胞组成性表达CD14和Toll样受体4 mRNA，暴露于脂多糖可使其增加。通过流式细胞术检测角质形成细胞CD14和Toll样受体4的细胞表面表达。脂多糖与角质形成细胞CD14和Toll样受体4结合导致细胞内Ca2+快速反应、核因子-κB核转位以及促炎细胞因子和趋化因子的分泌。这些结果对于我们理解皮肤对皮肤细菌感染的固有免疫具有重要意义。
