Berti G, Morsica R, Ventrelli I
Boll Ist Sieroter Milan. 1979 Jan 31;57(6):718-26.
The results of electroimmunodiffusion investigations with Reiter treponema antigen and syphilitic subject sera are presented. Counter-immunoelectrophoresis, rocked electrophoresis and crossed-electrophoresis studies have been performed. The test positivity is shown by the appearance of one ore two precipitates. No aspecificities were remarked with the latter two techniques: particular attention was devoted to the preparation of the treponema antigen in the attempt to eliminate the aspecificity which, sometimes, occurs above all with counter immuno-electrophoresis. Immuno-sorbents were also prepared to isolate IgG involved in this reaction: the latter turned out to be slow-migration IgG. Agarose-agar electroimmunoprecipitation between treponema antigens and sera of syphilitic subjects supplies possibilities for diagnosis and research in the field of treponema antigens and in the formation of antibodies in the syphilitic disease.
本文展示了用赖特氏密螺旋体抗原和梅毒患者血清进行免疫电泳研究的结果。进行了对流免疫电泳、摇床电泳和交叉电泳研究。出现一种或两种沉淀表明检测呈阳性。后两种技术未发现非特异性反应：特别关注了密螺旋体抗原的制备，试图消除有时在对流免疫电泳中尤其出现的非特异性反应。还制备了免疫吸附剂以分离参与该反应的IgG：结果发现后者是慢迁移IgG。密螺旋体抗原与梅毒患者血清之间的琼脂糖 - 琼脂免疫电沉淀为密螺旋体抗原领域的诊断和研究以及梅毒疾病中抗体的形成提供了可能性。
