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[从赖特密螺旋体中提取可溶性蛋白组分用作梅毒血清学诊断中的抗原]

[Extraction of the soluble protein fraction from Treponema Reiter for use as antigen in syphilis serodiagnosis].

作者信息

Georgescu M, Ionescu D

出版信息

Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol. 1978 Apr-Jun;23(2):93-100.

PMID:353957
Abstract

The present paper reports on two methods for extraction of the protein antigen from Treponema Reiter and shows the chemical and immunochemical characteristics of the extracts. Serologic testing of 1810 sera from syphilitic patients in various stages of the disease, from healthy subjects and from subjects with a false positive reaction to lipoid antigens, showed the value of the Reiter antigen to be equal to the cardiolipin antigens and those prepared from pathogenic Treponema. It is recommended to associate this antigen, in view of its qualities, to the cardiolipin tests used in the serodiagnosis of syphilis, especially in the laboratories that do not currently apply other treponema tests.

摘要

本文报道了从赖特密螺旋体中提取蛋白质抗原的两种方法,并展示了提取物的化学和免疫化学特性。对处于梅毒不同阶段的梅毒患者、健康受试者以及对类脂抗原呈假阳性反应的受试者的1810份血清进行血清学检测,结果表明赖特抗原的价值等同于心磷脂抗原以及由致病性密螺旋体制备的抗原。鉴于其特性,建议将这种抗原与梅毒血清诊断中使用的心磷脂试验相结合,特别是在目前不进行其他密螺旋体检测的实验室中。

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