Que Qiang Q, Winzeler Elizabeth A
Genomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Drive, San Diego, CA 92121, USA.
Funct Integr Genomics. 2002 Sep;2(4-5):193-8. doi: 10.1007/s10142-002-0057-3. Epub 2002 May 9.
One of the major goals for the post-genome era is determining of the function of proteins predicted in the genome sequence. In many organisms functional assignments have been the results of comparative sequencing, proteomics or expression profiling. In the yeast, Saccharomyces cerevisiae, however, the functional role of a gene can be tested directly by disrupting the gene and examining the phenotype of the mutant. Because precise targeted deletions can be easily constructed, it is also possible to systematically delete every gene in the genome. Here we describe recent progress in yeast genome-wide mutagenesis programs and the results produced from analyzing the mutants created by them.
后基因组时代的主要目标之一是确定基因组序列中预测的蛋白质的功能。在许多生物体中，功能分配是比较测序、蛋白质组学或表达谱分析的结果。然而，在酿酒酵母中，可以通过破坏基因并检查突变体的表型来直接测试基因的功能作用。由于可以轻松构建精确的靶向缺失，因此也有可能系统地删除基因组中的每个基因。在这里，我们描述了酵母全基因组诱变计划的最新进展以及分析由它们产生的突变体所得到的结果。
