Zagulski M, Herbert C J, Rytka J
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa.
Acta Biochim Pol. 1998;45(3):627-43.
The genome of the yeast Saccharomyces cerevisiae was sequenced by an international consortium of laboratories from Europe, Canada, the U.S.A. and Japan. This project is now finished and the complete sequence of the first eukaryotic genome was released to the public data bases in April 1996. An overview and preliminary analysis of the entire genome sequence was presented in a special issue of Nature in May 1997, entitled "The yeast genome directory". At its origin the Yeast Genome Sequencing Project provoked much debate and controversy; however, the final results obtained and the insights this has given us into the organisation and content of a eukaryotic genome have more than justified the expectations of the supporters of the project. The importance of genomic sequencing and analysis, especially of model organisms, is now widely accepted and this has resulted in the birth of the new science of genomics (Botstein & Cherry, 1997, Proc. Natl. Acad. Sci. U.S.A. 94, 5506). The information from gene and protein sequences ultimately lead to functional description of all genes. The main strategies describing possible ways to analyse the function of new genes that have been identified by systematic sequencing of Saccharomyces cerevisiae genome are described.
酿酒酵母的基因组由来自欧洲、加拿大、美国和日本的实验室国际联盟进行了测序。该项目现已完成，首个真核生物基因组的完整序列于1996年4月发布到公共数据库中。1997年5月，《自然》杂志的一期特刊发表了对整个基因组序列的概述和初步分析，特刊名为《酵母基因组目录》。酵母基因组测序项目从一开始就引发了诸多争论和争议；然而，所获得的最终结果以及它为我们提供的关于真核生物基因组的组织和内容的见解，完全证明了该项目支持者的期望是合理的。基因组测序和分析的重要性，尤其是对模式生物的测序和分析，现在已被广泛接受，这催生了新的基因组学科学（博特斯坦和彻里，1997年，《美国国家科学院院刊》94卷，5506页）。来自基因和蛋白质序列的信息最终会导向对所有基因的功能描述。本文描述了通过对酿酒酵母基因组进行系统测序而鉴定出的新基因功能分析的主要策略。
