Ide Takashi, Kushiro Masayo, Takahashi Yoko, Shinohara Kazuki, Cha Songhoon
Division of Food Functionality, National Food Research Institute, Tsukuba, Japan.
Metabolism. 2002 Sep;51(9):1191-7. doi: 10.1053/meta.2002.34036.
mRNA levels of the enzymes involved in taurine synthesis were compared among 12 rat tissues. Using a quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Northern-blotting, high levels of mRNA for cysteine dioxygenase were confirmed in the liver and kidney. Unexpectedly, the mRNA levels in epididymal and perirenal white adipose tissues and interscapular brown adipose tissue were remarkably high, at least compared with those observed in the liver and kidney. Cysteine dioxygenase mRNA levels in other tissues were very low. Using Northern blotting, significant amounts of cysteine sulfinic acid decarboxylase mRNA were detected in the liver, kidney, epididymal and perirenal white adipose tissues, and brown adipose tissue, but not in other tissues. Again, the mRNA levels of cysteine sulfinic acid decarboxylase in adipose tissues were comparable to or even higher than those in the liver and kidney. The activity of cysteine sulfinic acid decarboxylase in white and brown adipose tissues was 50% to 80% of that in the liver and much higher than the values observed in kidney, lung, and brain. However, the occurrence of cysteine dioxygenase activity in adipose tissues was not confirmed.
在12种大鼠组织中比较了参与牛磺酸合成的酶的mRNA水平。使用定量逆转录聚合酶链反应（RT-PCR）和Northern印迹法，证实肝脏和肾脏中半胱氨酸双加氧酶的mRNA水平较高。出乎意料的是，附睾和肾周白色脂肪组织以及肩胛间棕色脂肪组织中的mRNA水平非常高，至少与在肝脏和肾脏中观察到的水平相比是这样。其他组织中半胱氨酸双加氧酶的mRNA水平非常低。使用Northern印迹法，在肝脏、肾脏、附睾和肾周白色脂肪组织以及棕色脂肪组织中检测到大量的半胱氨酸亚磺酸脱羧酶mRNA，但在其他组织中未检测到。同样，脂肪组织中半胱氨酸亚磺酸脱羧酶的mRNA水平与肝脏和肾脏中的相当，甚至更高。白色和棕色脂肪组织中半胱氨酸亚磺酸脱羧酶的活性是肝脏中活性的50%至80%，远高于在肾脏、肺和脑中观察到的值。然而，未证实脂肪组织中存在半胱氨酸双加氧酶活性。
