Francisco C C, Chamberlain C S, Waldner D N, Wettemann R P, Spicer L J
Department of Animal Sciences, Oklahoma State University, Stillwater 74078-0425, USA.
J Dairy Sci. 2002 Jul;85(7):1738-51. doi: 10.3168/jds.S0022-0302(02)74248-3.
To determine the effect of feeding Propionibacteria on energy balance, milk yield, and composition, metabolites and hormones of early-lactating dairy cows, multiparous Holstein cows were individually fed a total mixed ration from -2 to 12 wk postpartum with no addition (control, n = 10) or with an additional 17 g of Propionibacteria culture daily (Treated, n = 9). Daily feed intake and milk production were recorded. Plasma cholesterol, nonesterified fatty acids (NEFA), leptin, insulin, glucose, insulin-like growth factor-I (IGF-I), IGF-binding proteins (IGFBP), and progesterone concentrations were measured up to twice weekly. Cows fed supplemental Propionibacteria had improved energy balance at wk 1 of lactation and had lower DMI per kg of body weight than control cows on wk 3 to 7, 10, and 12. Cows fed Propionibacteria had a greater percentage of milk protein and solids-not-fat and plasma NEFA concentrations than did control cows only at wk 1 of lactation. Treatment did not affect milk production or percentage of milk fat and lactose. Leptin levels were greater in treated than control cows throughout the study. Plasma glucose, insulin, cholesterol, IGFBP-3, and IGF-I concentrations were not affected by feeding Propionibacteria, but those variables increased with week postpartum. Plasma IGFBP-2 and IGFBP-5 levels decreased with week postpartum. Measures of reproductive and ovarian function did not differ between Propionibacteria-treated and control cows. Feeding Propionibacteria culture to transition and early lactating dairy cows may hold potential for improved milk protein production and metabolic efficiency during early lactation, without affecting reproductive function.
为了确定饲喂丙酸杆菌对初产奶牛能量平衡、产奶量、奶成分、代谢物及激素的影响，对经产荷斯坦奶牛进行了如下试验：从产后-2周到12周，给奶牛单独饲喂全混合日粮，一组不添加任何物质（对照组，n = 10），另一组每天额外添加17 g丙酸杆菌培养物（处理组，n = 9）。记录每日采食量和产奶量。每周最多测定两次血浆胆固醇、非酯化脂肪酸（NEFA）、瘦素、胰岛素、葡萄糖、胰岛素样生长因子-I（IGF-I）、IGF结合蛋白（IGFBP）和孕酮浓度。饲喂补充丙酸杆菌的奶牛在泌乳第1周时能量平衡得到改善，在第3至7周、第10周和第12周时，每千克体重的干物质采食量低于对照组奶牛。仅在泌乳第1周时，饲喂丙酸杆菌的奶牛乳蛋白和非脂固形物的百分比以及血浆NEFA浓度高于对照组奶牛。处理对产奶量或乳脂和乳糖百分比没有影响。在整个研究过程中，处理组奶牛的瘦素水平高于对照组奶牛。饲喂丙酸杆菌对血浆葡萄糖、胰岛素、胆固醇、IGFBP-3和IGF-I浓度没有影响，但这些变量随产后周数增加。血浆IGFBP-2和IGFBP-5水平随产后周数下降。丙酸杆菌处理组和对照组奶牛的生殖和卵巢功能指标没有差异。给围产期和初产奶牛饲喂丙酸杆菌培养物可能有助于提高泌乳早期的乳蛋白产量和代谢效率，而不影响生殖功能。
