台湾地区红细胞同种抗体抗-Mi(a)的免疫学特征
Immunological characterization of anti-Mi(a), a red blood cell alloantibody, in Taiwan.
作者信息
Lo S-C, Chang J-S, Lin S W-S, Lin D-T
机构信息
Department of Laboratory Medicine, National Taiwan University Hospital, 7 Chung-Shan South Road, Taipei 10018, Taiwan.
出版信息
Vox Sang. 2002 Aug;83(2):162-4. doi: 10.1046/j.1423-0410.2002.00212.x.
BACKGROUND AND OBJECTIVES
The red blood cell Mi III phenotype is prevalent in Asia, and its corresponding alloantibody, anti-Mi(a), has been reported to cause haemolytic transfusion reactions and haemolytic disease of the newborn. However, a complete picture of the immunological characteristics of anti-Mi(a) is still lacking. We therefore conducted a systematic study to evaluate the potential clinical significance of this antibody.
MATERIALS AND METHODS
From April 1999 to March 2000, we identified 60 sera containing anti-Mi(a) among pretransfusion samples at a teaching hospital in Taiwan. These antibodies were tested for immunoglobulin class, thermal range and activity in a monocyte monolayer assay.
RESULTS
Thirty-four (57%) of the antibodies were immunoglobulin M (IgM), and 15 retained their activity at 37 degrees C. Of those that were immunoglobulin G (IgG), 96% were of subclasses IgG1 and/or IgG3. Monocyte monolayer assay studies showed that 69% (18/26) of the IgG anti-Mi(a) sera were reactive.
CONCLUSIONS
Our study justifies the implementation of anti-Mi(a) screening in Taiwan.
背景与目的
红细胞Mi III表型在亚洲较为普遍，据报道，其相应的同种抗体抗-Mi(a)可引起溶血性输血反应及新生儿溶血病。然而，抗-Mi(a)的免疫学特征仍不完全清楚。因此，我们进行了一项系统研究，以评估该抗体的潜在临床意义。
材料与方法
1999年4月至2000年3月期间，我们在台湾一家教学医院的输血前样本中鉴定出60份含有抗-Mi(a)的血清。对这些抗体进行免疫球蛋白类别、热反应范围检测，并采用单核细胞单层试验检测其活性。
结果
34份（57%）抗体为免疫球蛋白M（IgM），15份在37℃时仍保持活性。在免疫球蛋白G（IgG）抗体中，96%为IgG1和/或IgG3亚类。单核细胞单层试验研究显示，69%（18/26）的IgG抗-Mi(a)血清呈反应性。
结论
我们的研究证明在台湾开展抗-Mi(a)筛查是合理的。
Zotero 插件