Rhoades Matthew W, Reinhart Brenda J, Lim Lee P, Burge Christopher B, Bartel Bonnie, Bartel David P
Whitehead Institute for Biomedical Research, 9 Cambridge Center, MA 02142, USA.
Cell. 2002 Aug 23;110(4):513-20. doi: 10.1016/s0092-8674(02)00863-2.
We predict regulatory targets for 14 Arabidopsis microRNAs (miRNAs) by identifying mRNAs with near complementarity. Complementary sites within predicted targets are conserved in rice. Of the 49 predicted targets, 34 are members of transcription factor gene families involved in developmental patterning or cell differentiation. The near-perfect complementarity between plant miRNAs and their targets suggests that many plant miRNAs act similarly to small interfering RNAs and direct mRNA cleavage. The targeting of developmental transcription factors suggests that many plant miRNAs function during cellular differentiation to clear key regulatory transcripts from daughter cell lineages.
我们通过识别具有近乎互补性的mRNA来预测14个拟南芥微小RNA（miRNA）的调控靶标。预测靶标内的互补位点在水稻中是保守的。在49个预测靶标中，34个是参与发育模式形成或细胞分化的转录因子基因家族的成员。植物miRNA与其靶标之间近乎完美的互补性表明，许多植物miRNA的作用类似于小干扰RNA，并指导mRNA的切割。对发育转录因子的靶向作用表明，许多植物miRNA在细胞分化过程中发挥作用，以清除子细胞谱系中的关键调控转录本。
