Bélanger Myriam, Sorenson Heather L, France Michelle K, Bowie Michael V, Barbet Anthony F, Breitschwerdt Edward B, Alleman A Rick
Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610, USA.
J Clin Microbiol. 2002 Sep;40(9):3506-8. doi: 10.1128/JCM.40.9.3506-3508.2002.
We determined the value of four serological assays for the diagnosis of canine monocytic ehrlichiosis by comparing them to the indirect fluorescent-antibody assay "gold standard." The specificity of Dip-S-Ticks was significantly lower than that of all of the other tests evaluated. The sensitivity of Dip-S-Ticks was significantly higher than that of Snap3Dx or the Snap Canine Combo. The sensitivity of the rMAP2 enzyme-linked immunosorbent assay (ELISA) was significantly higher than that of the Snap Canine Combo. The accuracy levels of the rMAP2 ELISA, Snap3Dx, Dip-S-Ticks, and Snap Canine Combo were 97.0, 89.8, 85.1, and 82.9%, respectively.
通过与间接荧光抗体检测这一“金标准”进行比较,我们确定了四种血清学检测方法在诊断犬单核细胞埃立克体病方面的价值。Dip - S - Ticks检测的特异性显著低于所有其他评估检测。Dip - S - Ticks检测的敏感性显著高于Snap3Dx或Snap犬类组合检测。rMAP2酶联免疫吸附测定(ELISA)的敏感性显著高于Snap犬类组合检测。rMAP2 ELISA、Snap3Dx、Dip - S - Ticks和Snap犬类组合检测的准确率分别为97.0%、89.8%、85.1%和82.9%。