Sosa-Gutierrez Carolina Guadalupe, Quintero Martinez Maria Teresa, Gaxiola Camacho Soila Maribel, Cota Guajardo Silvia, Esteve-Gassent Maria D, Gordillo-Pérez María-Guadalupe
Unidad de Investigacion Medica en Enfermedades Infecciosas y Parasitarias, Hospital de Pediatria, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, 07300 Mexico City, Mexico ; Departamento de Parasitologia, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de México, Mexico City, Mexico.
Departamento de Parasitologia, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de México, Mexico City, Mexico.
J Vet Med. 2013;2013:797019. doi: 10.1155/2013/797019. Epub 2013 Oct 24.
Ehrlichia canis is a rickettsial intracellular obligate bacterial pathogen and agent of canine monocytic ehrlichiosis. The prevalence of this disease in veterinary medicine can vary depending on the diagnostic method used and the geographic location. One hundred and fifty-two canine blood samples from six veterinary clinics and two shelters from Sinaloa State (Mexico) were analyzed in this study. All animals were suspected of having Canine Monocytic Ehrlichiosis (CME). The diagnostic methods used were the ELISA (Snap4Dx, IDEXX) together with blood smear and platelet count. From all dogs blood samples analyzed, 74.3% were positive to E. canis by ELISA and 40.1% were positive by blood smear. The sensitivity and specificity observed in the ELISA test were 78.8% and 86.7%. In addition, thrombocytopenia was presented in 87.6% of positive dogs. The predominant clinical manifestations observed were fever, anorexia, depression, lethargy, and petechiae. Consequently, this is the first report in which the morulae were visualized in the blood samples, and E. canis-specific antibodies were detected in dogs from Sinaloa, Northwest of Mexico.
犬埃立克体是一种立克次氏体细胞内专性细菌病原体，也是犬单核细胞埃立克体病的病原体。该疾病在兽医学中的流行率可能因所使用的诊断方法和地理位置而异。本研究分析了来自墨西哥锡那罗亚州六家兽医诊所和两个收容所的152份犬血样。所有动物均疑似患有犬单核细胞埃立克体病（CME）。所使用的诊断方法是酶联免疫吸附测定（Snap4Dx，IDEXX）以及血涂片和血小板计数。在所有分析的犬血样中，酶联免疫吸附测定显示74.3%的样本对犬埃立克体呈阳性，血涂片显示40.1%呈阳性。酶联免疫吸附测定试验观察到的敏感性和特异性分别为78.8%和86.7%。此外，87.6%的阳性犬出现血小板减少症。观察到的主要临床表现为发热、厌食、抑郁、嗜睡和瘀点。因此，这是第一份在血样中观察到桑葚体并在墨西哥西北部锡那罗亚州的犬中检测到犬埃立克体特异性抗体的报告。
