5-溴脱氧尿嘧啶核苷、神经D（nrd）和Hu的研究揭示了胚胎后斑马鱼前脑存在异常的非脑室神经发生。

BrdU-, neuroD (nrd)- and Hu-studies reveal unusual non-ventricular neurogenesis in the postembryonic zebrafish forebrain.

作者信息

Mueller Thomas, Wullimann Mario F

机构信息

FB 2, Brain Research Institute, University of Bremen, PO Box 330440, 28344 Bremen, Germany.

出版信息

Mech Dev. 2002 Sep;117(1-2):123-35. doi: 10.1016/s0925-4773(02)00194-6.

DOI:10.1016/s0925-4773(02)00194-6

PMID:12204253

Abstract

In the postembryonic zebrafish forebrain, subpial locations of neurogenesis do exist in the early cerebellar external granular layer, and--unusually among vertebrates--in the primordial pretectal (M1) and preglomerular (M2) Anlagen as shown here with 5-bromo-2'-deoxyuridine (BrdU)/Hu-immunocytochemistry and in situ hybridization of neuroD. An intermediate BrdU incubation time of 12-16 h reveals in addition to proliferative ventricularly located cells those in M1 and M2. This BrdU saturation-labeling shows--in conjunction with a Hu-assay demonstrating earliest neuronal differentiation--that proliferating cells in M1 and M2 represent neuronal progenitors. This is demonstrated by single BrdU-labeled and double BrdU-/Hu-labeled cells in these aggregates. Further, expression of NeuroD--a marker for freshly determined neuronal cells--confirms this unusual subpial postembryonic forebrain neurogenesis.

摘要

在胚胎后期的斑马鱼前脑中，神经发生的软膜下位置确实存在于早期小脑外颗粒层，而且——在脊椎动物中不同寻常的是——存在于原顶盖前区（M1）和前肾小球原基（M2），此处通过5-溴-2'-脱氧尿苷（BrdU）/Hu免疫细胞化学以及NeuroD原位杂交得以显示。12至16小时的中间BrdU孵育时间除了能显示位于脑室的增殖细胞外，还能显示M1和M2中的增殖细胞。这种BrdU饱和标记结合显示最早神经元分化的Hu检测表明，M1和M2中的增殖细胞代表神经元祖细胞。这些聚集体中单个BrdU标记细胞以及双BrdU-/Hu标记细胞证实了这一点。此外，NeuroD（一种用于新确定的神经元细胞的标志物）的表达证实了这种不同寻常的胚胎后期前脑软膜下神经发生。