Dodson Robin E, Shapiro David J
Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana 61801, USA.
Prog Nucleic Acid Res Mol Biol. 2002;72:129-64. doi: 10.1016/s0079-6603(02)72069-2.
The level of an mRNA in the cytoplasm represents a balance between the rate at which the mRNA precursor is synthesized in the nucleus and the rates of nuclear RNA processing and export and cytoplasmic mRNA degradation. Although most studies of gene expression have focused on gene transcription and in the area of eukaryotic mRNA degradation, but to provide a short general discussion of the importance of mRNA degradation and its regulation and a brief overview of recent findings and present knowledge. The overview is followed by a more in-depth discussion of one of the several pathways for mRNA degradation. We concentrate on the pathway for regulated mRNA degradation mediated by mRNA-binding proteins and endonucleases that cleave within the body of mRNAs. As a potential example of this type of control, we focus on the regulated degradation of the egg yolk precursor protein vitellogenin on the mRNA-binding protein vigilin and the mRNA endonuclease polysomal ribonuclease 1 (PMR-1).
细胞质中mRNA的水平代表了mRNA前体在细胞核中合成的速率与核RNA加工、输出以及细胞质中mRNA降解速率之间的平衡。尽管大多数基因表达研究都集中在基因转录以及真核mRNA降解领域，但本文将简要概述mRNA降解及其调控的重要性，并简要介绍近期的研究发现和现有知识。随后将对几种mRNA降解途径之一进行更深入的讨论。我们专注于由mRNA结合蛋白和核酸内切酶介导的、在mRNA内部进行切割的受调控mRNA降解途径。作为这种调控类型的一个潜在例子，我们重点研究卵黄前体蛋白卵黄生成素在mRNA结合蛋白vigilin和mRNA核酸内切酶多核糖体核糖核酸酶1（PMR-）作用下的受调控降解。 1 （PMR-1）。
需注意，原文中“polysomal ribonuclease 1 (PMR-1)”后面多了个“-”，翻译时已按正确内容翻译。
