使用流动注射毛细管电泳系统在线转换与测定青蒿素。
On-line conversion and determination of artemisinin using a flow-injection capillary electrophoresis system.
作者信息
Chen Hong Li, Wang Ke Tai, Pu Qiao Sheng, Chen Xing Guo, Hu Zhi De
机构信息
Department of Chemistry, Lanzhou University, Lanzhou, PR China.
出版信息
Electrophoresis. 2002 Sep;23(17):2865-71. doi: 10.1002/1522-2683(200209)23:17<2865::AID-ELPS2865>3.0.CO;2-Q.
A novel, rapid, and simple capillary electrophoresis (CE) method has been developed for the determination of antimalarial artemisinin by on-line treatment with alkaline. By on-line reaction, artemisinin was automatically and reproducibly converted to the strongly UV-absorbing compound, Q292, by treating it with 0.20 mol/L NaOH solution for 3 min at 40 degrees C. Analysis was carried out in less than 12 min after conversion of artemisinin in a flow injection (FI) system that was coupled to CE equipment via a split-flow interface cell, and a sampling frequency of 8 h(-1) is achievable. The on-line conversion method has been applied to the determination of artemisinin in the traditional Chinese herbal drug Artemisia annua L., and the results are satisfactory.
已开发出一种新颖、快速且简单的毛细管电泳（CE）方法，用于通过碱性在线处理来测定抗疟药物青蒿素。通过在线反应，在40℃下用0.20 mol/L NaOH溶液处理青蒿素3分钟，青蒿素可自动且可重复地转化为强紫外吸收化合物Q292。在通过分流接口池与CE设备相连的流动注射（FI）系统中，青蒿素转化后不到12分钟即可进行分析，采样频率可达8 h⁻¹。该在线转化方法已应用于中药青蒿中青蒿素的测定，结果令人满意。
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