Zhou Ao-Ao, Zheng Wen-Xin, Ge Fa-Huan
National Engineering Research Center for Modernization of Extraction and Separation Process of TCM, Guangzhou 510240, China.
Zhong Yao Cai. 2006 Mar;29(3):242-5.
A HPLC method was first established to determine artemisinin in Artemisia annuna by HPLC-ELSD. Artemisinin in Herba of Artemisia annuna from different places was determined by this new method.
The method utilized MeOH-H2O (75: 25) as mobile phase with flow rate of 1 ml/min, C18 (250 x 4.6 mm, 5 microm) column. The temperature of detector was 40 degrees C, the pressure of N2 was 3.5 bar, and gain value was 9.
The calibration curves were linear within the range of 1 - 5 microg, and the average recovery was 99.33% (RSD = 1.97%).
The method was rapid, convenient, and accurate. It can be used for the quality control of this herbal medicine.
首次建立了高效液相色谱-蒸发光散射检测法（HPLC-ELSD）测定黄花蒿中青蒿素的含量，并采用该新方法测定了不同产地黄花蒿药材中的青蒿素。
该方法以甲醇-水（75:25）为流动相，流速为1 ml/min，采用C18（250×4.6 mm，5 µm）色谱柱。检测器温度为40℃，氮气压力为3.5 bar，增益值为9。
校准曲线在1 - 5 μg范围内呈线性，平均回收率为99.33%（相对标准偏差RSD = 1.97%）。
该方法快速、简便、准确，可用于该中药材的质量控制。
