Bilia A R, Melillo de Malgalhaes P, Bergonzi M C, Vincieri F F
Department of Pharmaceutical Sciences, University of Florence, Via U. Schiff 6, 50019 Sesto Fiorentino-Florence, Italy.
Phytomedicine. 2006 Jul;13(7):487-93. doi: 10.1016/j.phymed.2006.01.008. Epub 2006 Feb 20.
Artemisia annua L. (Qinghao) is a promising and potent antimalarial herbal drug. This activity has been ascribed to its component artemisinin, a sesquiterpene lactone that is very effective against drug-resistant Plasmodium species with a low toxicity. Our studies indicate that several flavonoids of A. annua can promote and enhance the reaction of artemisinin with hemin. These data are in good agreement with previous investigations on the in vitro potentiation of antimalarial activity of artemisinin by such flavonoids. As a consequence, in view of a possible use of the phytocomplex rather than pure artemisinin, an HPLC/DAD/MS method is proposed for the simultaneous detection and quantification of both flavonoids and artemisinin. Different extracts, obtained from two different herbal drugs, a commercial sample and a selected cultivar, were analyzed in order to determine which solvents provide the best yields of both artemisinin and flavonoids. Qualitative and quantitative results obtained using an HPLC method are described, which will be useful for developing highly effective herbal drug preparations.
青蒿是一种很有前景且高效的抗疟草药。这种活性归因于其成分青蒿素，一种倍半萜内酯，对耐药疟原虫种类非常有效且毒性低。我们的研究表明，青蒿中的几种黄酮类化合物可促进和增强青蒿素与血红素的反应。这些数据与先前关于此类黄酮类化合物在体外增强青蒿素抗疟活性的研究结果高度一致。因此，鉴于可能使用植物复合物而非纯青蒿素，提出了一种HPLC/DAD/MS方法，用于同时检测和定量黄酮类化合物和青蒿素。分析了从两种不同草药、一个商业样品和一个选定品种中获得的不同提取物，以确定哪种溶剂能使青蒿素和黄酮类化合物的产量最高。描述了使用HPLC方法获得的定性和定量结果，这将有助于开发高效的草药制剂。
