Enbom Malin, Urassa Willy, Massambu Charles, Thorstensson Rigmor, Mhalu Fred, Linde Annika
Department of Virology, Swedish Institute for Infectious Disease Control and Microbiology and Tumor Biology Center, Karolinska Institute, Sweden.
J Med Virol. 2002 Oct;68(2):264-7. doi: 10.1002/jmv.10183.
Human herpesvirus 8 (HHV-8) is associated with Kaposi's sarcoma. There is a high seroprevalence of HHV-8 in several African countries, but the transmission route is not known definitively. In this study 174 serum samples from blood donors in Tanzania were examined by immunofluorescence assays detecting antibodies to latent and lytic HHV-8 antigens. Real-time polymerase chain reaction was used for detection and quantification of HHV-8 DNA in serum. In all, 83/174 (48%) of the subjects had antibodies to latent or lytic antigens. Forty (23%) had antibodies to both antigens and of those eight (20%) had detectable HHV-8 DNA in serum. HHV-8 DNA load correlated with antibody titres to lytic, but not latent, HHV-8 antigens. This supports the usefulness of anti-lytic antibodies in HHV-8 serology and suggests that transmission of HHV-8 by blood contact could be of importance in this region.
人类疱疹病毒8型（HHV-8）与卡波西肉瘤相关。在几个非洲国家，HHV-8的血清阳性率很高，但确切的传播途径尚不清楚。在本研究中，通过免疫荧光测定法检测针对潜伏性和溶解性HHV-8抗原的抗体，对坦桑尼亚献血者的174份血清样本进行了检测。采用实时聚合酶链反应检测和定量血清中的HHV-8 DNA。总共，174名受试者中有83名（48%）具有针对潜伏性或溶解性抗原的抗体。40名（23%）同时具有针对两种抗原的抗体，其中8名（20%）血清中可检测到HHV-8 DNA。HHV-8 DNA载量与针对溶解性而非潜伏性HHV-8抗原的抗体滴度相关。这支持了抗溶解性抗体在HHV-8血清学中的有用性，并表明通过血液接触传播HHV-8在该地区可能很重要。
