Bergallo Massimiliano, Costa Cristina, Margio Samuela, Sidoti Francesca, Re Daniela, Segoloni Giuseppe P, Cavallo Rossana
Dipartimento di Sanità Pubblica e Microbiologia, Laboratorio di Virologia, Università di Torino, Italy, Università di Torino, Via Santena 9, 10126 Turin, Italy.
Nephrol Dial Transplant. 2007 Jun;22(6):1757-61. doi: 10.1093/ndt/gfm056. Epub 2007 Mar 12.
Human herpes virus 8 (HHV-8) infection is associated with Kaposi sarcoma (KS) and other neoplastic and non-neoplastic manifestations. A strong association between HHV-8 and KS has been evidenced in transplant recipients, particularly kidney recipients.
We have investigated the HHV-8 seroprevalence by an immunoenzymatic assay in 408 patients awaiting kidney transplantation and in the corresponding 356 donors; moreover, we have tested for the presence of HHV-8 DNA by nested PCR in available serum samples of the same graft recipients at 6, 12 and >18 months post-transplantation (overall 156 specimens). Associated factors, such as age, sex, area of residence, potential for HHV-8 transmission via organ transplantation and development of KS were also investigated.
Twenty (4.9%) of 408 patients and 7 (1.9%) of 356 donors were seropositive. HHV-8 seropositive patients were on average about 6 years older than seronegative individuals. No difference in prevalence by gender was found. Considering the area of residence of seropositive patients, 4/161 (2.48%) were resident in Piemonte vs 16/247 (6.47%) in other areas of Italy (P = n.s.). During the follow-up post-transplantation, HHV-8 DNA was found only in four patients who were seropositive before transplantation, in three cases the corresponding donor was seronegative, in one the corresponding donor was also seropositive and the recipient developed KS. At >18 months post-transplantation, two patients were HHV-8 DNA positive, both were seronegative pre-transplantation and their corresponding donors were seronegative.
HHV-8 seroprevalence in the Piemonte region seems to be low, also in a population of kidney transplant recipients. Based on our data, it does not seem that the immunosuppressive regimen favours the reactivation of HHV-8. Our results do not suggest the possibility of HHV-8 transmission via organ transplantation. Incidence of KS among HHV-8 seropositive patients was very low.
人类疱疹病毒8型（HHV-8）感染与卡波西肉瘤（KS）及其他肿瘤性和非肿瘤性表现相关。HHV-8与KS之间的强关联已在移植受者中得到证实，尤其是肾移植受者。
我们采用免疫酶法对408例等待肾移植的患者及相应的356例供者进行了HHV-8血清阳性率调查；此外，我们通过巢式PCR检测了同一批移植受者在移植后6个月、12个月和超过18个月时（共156份标本）可用血清样本中HHV-8 DNA的存在情况。还调查了相关因素，如年龄、性别、居住地区、HHV-早发症状8型通过器官移植传播的可能性以及KS的发生情况。
408例患者中有20例（4.9%）血清阳性，356例供者中有7例（1.9%）血清阳性。HHV-8血清阳性患者的平均年龄比血清阴性患者大6岁左右。未发现性别患病率差异。考虑血清阳性患者的居住地区，161例中有4例（2.48%）居住在皮埃蒙特，而意大利其他地区为16/247例（6.47%）（P = 无显著差异）。在移植后的随访中，仅在4例移植前血清阳性的患者中发现了HHV-8 DNA，其中3例相应供者血清阴性，1例相应供者也血清阳性且受者发生了KS。移植后超过18个月时，2例患者HHV-8 DNA阳性，两者移植前均血清阴性且相应供者血清阴性。
皮埃蒙特地区的HHV-8血清阳性率似乎较低，肾移植受者群体中也是如此。根据我们的数据，免疫抑制方案似乎并未促进HHV-8的再激活。我们的结果未提示HHV-8通过器官移植传播的可能性。HHV-8血清阳性患者中KS的发病率非常低。
