Hudson Lola C, Vahlenkamp Thomas W, Colby Brenda, Meeker Rick B
Department of Anatomy, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
Contemp Top Lab Anim Sci. 2002 Sep;41(5):30-2.
We needed an effective technique for obtaining cerebrospinal fluid (CSF) from young (2- to 18-week-old) kittens. Standard veterinary technique was not suitable, so we adapted a previously published technique for rats. We first established an effective isoflurane-only anesthetic protocol for young kittens. After inhalant anesthesia, the kittens were positioned on a supporting platform to gain flexion of the head and neck. A micromanipulator was used to hold and slowly advance the collection needle. At the time this report was written, we had collected a total of 33 samples from eight kittens without causing apparent neurologic deficits. Correct positioning of the animal and collection needle was critical for success. This procedure enabled the collection of approximately 0.5 ml CSF from kittens younger than 12 weeks and larger volumes from older kittens.
我们需要一种有效的技术来从幼猫（2至18周龄）获取脑脊液（CSF）。标准的兽医技术并不适用，因此我们改编了一种先前发表的针对大鼠的技术。我们首先为幼猫建立了一种仅使用异氟烷的有效麻醉方案。吸入麻醉后，将幼猫放置在支撑平台上以使头颈部弯曲。使用显微操作器固定并缓慢推进采集针。在撰写本报告时，我们已从八只幼猫中总共采集了33个样本，且未造成明显的神经功能缺损。动物和采集针的正确定位是成功的关键。该程序能够从12周龄以下的幼猫中采集约0.5毫升脑脊液，从年龄较大的幼猫中采集更多量的脑脊液。
