Ujihara Noriko, Sakka Yumiko, Takeda Masanobu, Hirayama Michiyo, Ishii Akiko, Tomonaga Osamu, Babazono Tetsuya, Takahashi Chieko, Yamashita Katsuko, Iwamoto Yasuhiko
Diabetes Center and Institute of Geriatrics, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Diabetes Res Clin Pract. 2002 Nov;58(2):109-14. doi: 10.1016/s0168-8227(02)00134-1.
To investigate the association of oxidized low-density lipoprotein (ox-LDL) with the development of diabetic nephropathy, plasma levels of ox-LDL were measured in 70 patients with type 2 diabetes mellitus. A sandwich enzyme-linked immunoadsorbent assay (ELISA) using the mouse monoclonal antibody FOH1a/DLH3, which specifically recognizes oxidized phosphatidylcholine, and a horseradish peroxidase (HRP)-labeled goat anti-human apolipoprotein B IgG was used to measure ox-LDL levels. The mean age of the patients was 57.0+/-1 3.4 years, and the mean duration of diabetes was 13.4+/-8.5 years. Plasma ox-LDL levels were similar in patients with normoalbuminuria (13.7+/-3.9 U/ml), patients with microalbuminuria (12.8+/-3.9 U/ml), and normal controls (12.5+/-4.2 U/ml). However, the plasma ox-LDL level in patients with macroalbuminuria (16.8+/-7.5 U/ml) was significantly higher than those in the other groups (P<0.05). Hemoglobin A1c (HbA1c) levels were similar in diabetic patients with normoalbuminuria (8.2+/-2.2%), microalbuminuria (7.8+/-1.3%), or macroalbuminuria (7.2+/-1.4%). There was no significant correlation between the ox-LDL level and the HbA1c level. The significantly elevated plasma ox-LDL levels in patients with macroalbuminuria suggest that ox-LDL may play an important role in the progression of diabetic nephropathy.
为了研究氧化型低密度脂蛋白（ox-LDL）与糖尿病肾病发生发展的关系，对70例2型糖尿病患者的血浆ox-LDL水平进行了检测。采用夹心酶联免疫吸附测定法（ELISA），使用特异性识别氧化磷脂酰胆碱的小鼠单克隆抗体FOH1a/DLH3和辣根过氧化物酶（HRP）标记的山羊抗人载脂蛋白B IgG来测定ox-LDL水平。患者的平均年龄为57.0±13.4岁，平均糖尿病病程为13.4±8.5年。正常白蛋白尿患者（13.7±3.9 U/ml）、微量白蛋白尿患者（12.8±3.9 U/ml）和正常对照组（12.5±4.2 U/ml）的血浆ox-LDL水平相似。然而，大量白蛋白尿患者的血浆ox-LDL水平（16.8±7.5 U/ml）显著高于其他组（P<0.05）。正常白蛋白尿（8.2±2.2%）、微量白蛋白尿（7.8±1.3%）或大量白蛋白尿（7.2±1.4%）的糖尿病患者糖化血红蛋白（HbA1c）水平相似。ox-LDL水平与HbA1c水平之间无显著相关性。大量白蛋白尿患者血浆ox-LDL水平显著升高表明ox-LDL可能在糖尿病肾病进展中起重要作用。
