毛细管电泳中金属离子的高压非接触式电导检测

High-voltage contactless conductivity detection of metal ions in capillary electrophoresis.

作者信息

Tanyanyiwa Jatisai, Hauser Peter C

机构信息

The University of Basel, Department of Chemistry, Basel, Switzerland.

出版信息

Electrophoresis. 2002 Nov;23(21):3781-6. doi: 10.1002/1522-2683(200211)23:21<3781::AID-ELPS3781>3.0.CO;2-L.

DOI:10.1002/1522-2683(200211)23:21<3781::AID-ELPS3781>3.0.CO;2-L

PMID:12432541

Abstract

The detection of alkali, alkaline earth and heavy metal ions with a high-voltage capacitively coupled contactless conductivity detector (HV-C(4)D) was investigated. Eight alkali, alkaline earth metal ions and ammonium could be separated in less than 4 min with detection limits in the order of 5 x 10(-8) M. The heavy metals Mn2+, Pb2+, Cd2+ Fe2+, Zn2+, Co2+, Cu2+ and Ni2+ could also be successfully resolved with a 10 mM 2-(N-morpholino)ethanesulfonic acid/DL-histidine (MES/His)-buffer. Zn2+, Co2+, Cu2+ and Ni2+ showed an indirect response. The detection limits for the heavy metals were determined to range from about 1 to 5 microM.

摘要

研究了使用高压电容耦合非接触式电导检测器（HV-C(4)D）检测碱金属、碱土金属和重金属离子。八种碱金属、碱土金属离子和铵离子能够在不到4分钟内分离，检测限约为5×10(-8) M。重金属离子Mn2+、Pb2+、Cd2+、Fe2+、Zn2+、Co2+、Cu2+和Ni2+也能够在10 mM 2-(N-吗啉代)乙磺酸/DL-组氨酸（MES/His）缓冲液中成功分离。Zn2+、Co2+、Cu2+和Ni2+呈现间接响应。重金属的检测限确定在约1至5 microM范围内。