Wagner B, Veckenstedt A, Wagner M
Zentralbl Bakteriol Orig A. 1975 Dec;233(4):431-9.
Sindbis virus infected L cells were examined by immunoferritin technique at different times after infection. Initially virus-specific antigens appeared diffusely distributed in the cytoplasm and at the surface of vacuoles. Later on, both labelled nucleocapsids surrounding vacuoles and mature virus within their lumen were demonstrated. Maturation of virus occurred predominantly at the periphery of cells, and that mainly by budding from cell processes. The appearance of big crystals consisting of nucleocapsids is a typical event of late stages of infection. These findings are compared with corresponding fluorescent antibody results.
用免疫铁蛋白技术在感染后的不同时间检查辛德毕斯病毒感染的L细胞。最初，病毒特异性抗原呈弥漫性分布于细胞质和液泡表面。后来，显示出围绕液泡的标记核衣壳以及其腔内的成熟病毒。病毒成熟主要发生在细胞周边，主要通过从细胞突起出芽进行。由核衣壳组成的大晶体的出现是感染后期的典型事件。将这些发现与相应的荧光抗体结果进行比较。
