在胎羊肾（FLK）上培养的牛白血病病毒（BLV）的形态发生：一项超微结构研究。
The morphogenesis of BLV (bovine leukemia virus) cultivated on FLK (fetal lamb kidney): an ultrastructural study.
作者信息
Filice G, Carnevale G, Lanzarini P, Orsolini P, Soldini L, Trespi G, Cereda P M
机构信息
Department of Infectious Diseases, University of Pavia, I.R.C.C.S., San Matteo Viale Golgi, Italy.
出版信息
Microbiologica. 1988 Jan;11(1):1-5.
A new cell line, obtained by co-cultivation of fetal lamb kidney cells and lymphocytes collected from an adult calf affected by enzootic bovine leukemia, was studied for bovine leukemia virus (BLV) morphogenesis. In this new cell line, called FLK-BLV, persistently infected with BLV, we identified extracellular and intracellular BLV particles. We never observed "budding" particles along the cell surface, and therefore assumed it was a new BLV maturation process in the cell vacuoles. In fact we found mature and non-mature particles connected with the cell-membrane system or cellular debris within vacuoles. We suggest that the viral envelope could be supplied by vacuole membrane. In our samples we also observed a cytopathic effect with syncytia formations similar to those observed on other BLV-producing cell lines.
通过将胎羊肾细胞与从患地方流行性牛白血病的成年小牛采集的淋巴细胞共同培养获得了一种新的细胞系，对其进行了牛白血病病毒（BLV）形态发生的研究。在这个名为FLK - BLV的新细胞系中，持续感染了BLV，我们鉴定出了细胞外和细胞内的BLV颗粒。我们从未在细胞表面观察到“出芽”颗粒，因此推测这是细胞液泡中一种新的BLV成熟过程。事实上，我们发现成熟和未成熟颗粒与液泡内的细胞膜系统或细胞碎片相连。我们认为病毒包膜可能由液泡膜提供。在我们的样本中，还观察到了细胞病变效应，形成了类似于在其他产生BLV的细胞系中观察到的多核巨细胞。
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