Morice William G, Kurtin Paul J, Myers Jeffrey L
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
Am J Clin Pathol. 2002 Sep;118(3):391-8. doi: 10.1309/PMR7-7XLY-F10U-4V1Q.
Paraffin-embedded lung wedge biopsy specimens from 14 patients with pulmonary lymphomatoid granulomatosis (LYG) were analyzed using immunoperoxidase stains specific for T cell- and natural killer cell-associated antigens. Nine cases had a minor population of CD20+ large B-cells (B-cell LYG) amidst a background of CD3- and betaF1-immunoreactive T cells. In 8 of the 9 B-cell LYG cases, the majority of the background T lymphocytes had a cytotoxic phenotype as defined by the expression of CD8 and the cytotoxic granule proteins TIA-1 (granule membrane protein 17) and granzyme B. Five cases lacked CD20+ large cells and, instead, showed predominantly CD3+ and betaF1 + T cells (T-cell LYG). Whereas the small, medium, and large atypical lymphocytes were all positive for CD3 and betaF1 in the T-cell LYG cases, immunoreactivity for CD8, TIA-1, and granzyme B was limited to the small lymphocytes, with a distribution indistinguishable from that seen in B-cell LYG. These findings indicate that LYG is composed of a heterogeneous group of lymphoproliferative disorders that share, as unifying features, a relative paucity of neoplastic cells and a prominent reactive infiltrate rich in cytolytic lymphocytes.
使用针对T细胞和自然杀伤细胞相关抗原的免疫过氧化物酶染色法，对14例肺淋巴瘤样肉芽肿病（LYG）患者的石蜡包埋肺楔形活检标本进行了分析。9例在CD3和βF1免疫反应性T细胞背景中有少量CD20 +大B细胞（B细胞LYG）。在9例B细胞LYG病例中的8例中，根据CD8以及细胞毒性颗粒蛋白TIA-1（颗粒膜蛋白17）和颗粒酶B的表达定义，大多数背景T淋巴细胞具有细胞毒性表型。5例缺乏CD20 +大细胞，取而代之的是主要显示CD3 +和βF1 + T细胞（T细胞LYG）。在T细胞LYG病例中，小、中、大非典型淋巴细胞均对CD3和βF1呈阳性，而CD8、TIA-1和颗粒酶B的免疫反应性仅限于小淋巴细胞，其分布与B细胞LYG中所见的分布无法区分。这些发现表明，LYG由一组异质性淋巴增殖性疾病组成，这些疾病具有共同特征，即肿瘤细胞相对较少，以及富含溶细胞性淋巴细胞的显著反应性浸润。
