Fayrer-Hosken R A, Barber M R, Crane M, Collins T, Hodgden R
Department of Large Animal Medicine and Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens 30602-7385, USA.
Reprod Suppl. 2002;60:125-9.
Thirteen deer (Odocoileus virginianus) and three goats (Capra hircus) were given an initial immunization of 300 microg porcine zona pellucida (PZP) combined with 50 mg synthetic trehalose dicorynomycolate ml(-1) (STDCM) in drakeol i.m. in the semitendinosus and semimembranosus muscles. This immunization was followed by two booster injections of 300 microg PZP, 2 weeks apart. The vaccinations were made from the same purified batch of PZP and lot of adjuvant. All the doses were made at the same time and injected on the same day. The immune response was quantified by measuring serum anti-PZP IgG antibody concentration by ELISA. The results showed that the mean serum IgG concentration of the deer increased from 0.000 +/- 0.003 to 0.083 +/- 0.023 absorbance units, whereas in the goats the mean IgG concentration increase was from 0.044 +/- 0.019 to 1.245 +/- 0.774 absorbance units. Both the goats and deer showed a significant increase in IgG concentration (P < 0.05) after the final booster injection compared with preimmune serum. The final goat IgG concentration was significantly (P < 0.05) higher than that of the deer. If PZP and Freund's adjuvant are administered to deer, immunocontraception is achieved, but the IgG concentration in the deer in this study did not appear to be compatible with immunocontraception.
给13只鹿（弗吉尼亚鹿）和3只山羊肌肉注射了初始疫苗，疫苗为300微克猪透明带（PZP）与50毫克合成海藻糖二分枝菌酸酯（STDCM）的混合物，溶剂为轻质液体石蜡，注射部位为半腱肌和半膜肌。首次免疫后，间隔两周进行两次300微克PZP的加强注射。所有疫苗均来自同一批次纯化的PZP和同一批次佐剂。所有剂量均同时配制并在同一天注射。通过酶联免疫吸附测定（ELISA）测量血清抗PZP IgG抗体浓度来量化免疫反应。结果显示，鹿的血清IgG平均浓度从0.000±0.003吸光度单位增加到0.083±0.023吸光度单位，而山羊的血清IgG平均浓度从0.044±0.019吸光度单位增加到1.245±0.774吸光度单位。与免疫前血清相比，在最后一次加强注射后，山羊和鹿的IgG浓度均显著增加（P<0.05）。山羊的最终IgG浓度显著高于鹿（P<0.05）。如果给鹿注射PZP和弗氏佐剂，可实现免疫避孕，但本研究中鹿的IgG浓度似乎与免疫避孕不相容。
