Schaefer Didier G
Institut d'Ecologie, Laboratoire de Phytogénétique Cellulaire, Bâtiment de Biologie, Université de Lausanne, CH-1015 Lausanne, Switzerland.
Annu Rev Plant Biol. 2002;53:477-501. doi: 10.1146/annurev.arplant.53.100301.135202.
The potential of moss as a model system to study plant biology is associated with their relatively simple developmental pattern that nevertheless resembles the basic organization of the body plan of land plants, the direct access to cell-lineage analysis, their similar responses to plant growth factors and environmental stimuli as those observed in other land plants, and the dominance of the gametophyte in the life cycle that facilitates genetic approaches. Transformation studies in the moss Physcomitrella patens have revealed a totally unique feature for plants, i.e., that foreign DNA sequences integrate in the genome preferentially at targeted locations by homologous recombination, enabling for the first time in plants the application of the powerful molecular genetic approaches used routinely in bacteria, yeast, and since 1989, the mouse embryonic stem cells. This article reviews our current knowledge of Physcomitrella patens transformation and its unique suitability for functional genomic studies.
苔藓作为研究植物生物学的模式系统，其潜力与其相对简单的发育模式有关，这种发育模式尽管如此却类似于陆地植物身体结构的基本组织，便于直接进行细胞谱系分析，它们对植物生长因子和环境刺激的反应与其他陆地植物相似，并且配子体在生命周期中占主导地位，这有利于采用遗传学方法。对小立碗藓的转化研究揭示了植物一个完全独特的特征，即外源DNA序列通过同源重组优先整合到基因组的靶向位置，这使得在植物中首次能够应用细菌、酵母以及自1989年以来在小鼠胚胎干细胞中常规使用的强大分子遗传学方法。本文综述了我们目前对小立碗藓转化及其在功能基因组学研究中独特适用性的认识。
