Cubero J, van der Wolf J, van Beckhoven J, López M M
Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, Cra Moncada Náquera Km 4,5, Apartado Oficial 46113 Moncada, Valencia, Spain.
J Microbiol Methods. 2002 Nov;51(3):387-92. doi: 10.1016/s0167-7012(02)00153-7.
The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown gall diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified.
添加适当浓度的内部控制（IC）能够识别根癌土壤杆菌检测中的假阴性，并提高用于冠瘿诊断的PCR的可靠性。如果提取的DNA质量足够高以进行扩增，那么从根癌土壤杆菌中共扩增IC和靶序列可确保在每个PCR反应中至少获得一个扩增产物。
