Cubero J, Martínez M C, Llop P, López M M
Instituto Valenciano de Investigaciones Agrarias, Moncada, Valencia, Spain.
J Appl Microbiol. 1999 Apr;86(4):591-602. doi: 10.1046/j.1365-2672.1999.00700.x.
A simple PCR protocol was developed for identifying Agrobacterium as the causal agent of the tumours produced by this bacterium in plant material. The sensitivity of this method was compared with that of bacterial isolation using common and selective media with a previous enrichment step. More than 200 samples from tumours of naturally infected and inoculated plants from several hosts including almond, peach x almond hybrids, apricot, rose, tobacco, tomato, raspberry, grapevine and chrysanthemum, were analysed by both methods. PCR was the most efficient method for detecting the bacterial aetiology of the plant tumours. Agrobacterium tumefaciens was better detected in crown and root tumours than in aerial tumours with all the methods assayed in inoculated plants. A comparison between the efficiency of the diagnosis by analysing pieces from the external and internal part of the tumour showed no differences between them.
开发了一种简单的聚合酶链反应（PCR）方案，用于鉴定根癌土壤杆菌是该细菌在植物材料中产生肿瘤的致病因子。将该方法的灵敏度与使用普通和选择性培养基并经过预富集步骤的细菌分离方法的灵敏度进行了比较。通过这两种方法对来自包括杏仁、桃×杏仁杂交种、杏、玫瑰、烟草、番茄、覆盆子、葡萄和菊花在内的几种宿主的自然感染和接种植物肿瘤的200多个样本进行了分析。PCR是检测植物肿瘤细菌病因最有效的方法。在接种植物中使用的所有方法中，根癌土壤杆菌在冠部和根部肿瘤中比在地上肿瘤中更容易检测到。通过分析肿瘤外部和内部部分的切片来比较诊断效率，结果显示两者之间没有差异。
