Nakada Tsutomu, Watanabe Kenji, Jin Guang-bi, Triizuk Kazuo, Hanawa Toshihiko
Oriental Medicine Research Center of the Kitasato Institute, Japan.
Am J Chin Med. 2002;30(2-3):215-23. doi: 10.1142/S0192415X0200034X.
Ninjin-youei-to (NYT; ren-shen-yang-rong-tang in Chinese) is a traditional herbal formula, which is widely used in Japan, Korea and China to modulate physiological immunity. The effects of oral administration of NYT on cytokine production from splenocytes were investigated in both C57BL/6 and BALB/c mice in which Th1 and Th2 were dominant, respectively. Splenocytes from C57BL/6 and BALB/c mice, which took NYT orally forfour weeks, were cultured with anti-mouse CD3 mAb, and the supernatant was examined for cytokine production using enzyme-linked immunosorbent assay (ELISA). Administration of NYT to C57BL/6 mice, increased the production of interleukin-4 (IL-4) significantly, and slightly decreased interferon-y (IFN-gamma) production from splenocytes. In contrast, the same treatment significantly increased IFN-gamma secretion from splenocytes of BALB/c mice. No remarkable changes of IL-12 production from splenocytes were observed in either strain of mice. These results suggest that oral administration of NYT ameliorates the excessive inclination of Th1 and Th2 type cytokine production, and NYT may provide a beneficial effects for the treatment of diseases caused by a skewed Th1-Th2 balance in the immune system.
人参养荣汤（NYT；中文为“人参养荣汤”）是一种传统中药配方，在日本、韩国和中国被广泛用于调节生理免疫。分别在以Th1和Th2占主导的C57BL/6和BALB/c小鼠中研究了口服NYT对脾细胞细胞因子产生的影响。给口服NYT四周的C57BL/6和BALB/c小鼠的脾细胞用抗小鼠CD3单克隆抗体培养，并用酶联免疫吸附测定（ELISA）检测上清液中的细胞因子产生情况。给C57BL/6小鼠服用NYT可显著增加白细胞介素-4（IL-4）的产生，并使脾细胞产生的干扰素-γ（IFN-γ）略有减少。相反，相同处理显著增加了BALB/c小鼠脾细胞中IFN-γ的分泌。在两种品系的小鼠中均未观察到脾细胞产生IL-12有明显变化。这些结果表明，口服NYT可改善Th1和Th2型细胞因子产生的过度倾向，并且NYT可能对治疗由免疫系统中Th1-Th2平衡失调引起的疾病有有益作用。
