Belkhodja R., Morales F., Abadia A., Gomez-Aparisi J., Abadia J.
Departamento de Nutricion Vegetal, Estacion Experimental de Aula Dei, Consejo Superior de Investigaciones Cientificas, Apdo. 202, 50080 Zaragoza, Spain (R.B., F.M., A.A., J.A.).
Plant Physiol. 1994 Feb;104(2):667-673. doi: 10.1104/pp.104.2.667.
The application of chlorophyll fluorescence measurements to screening barley (Hordeum vulgare L.) genotypes for salinity tolerance has been investigated. Excised barley leaves were cut under water and incubated with the cut end immersed in water or in a 100-mM NaCl solution, either in the dark or in high light. Changes in rapid fluorescence kinetics occurred in excised barley leaves exposed to the saline solution only when the incubation was carried out in the presence of high light. Fluorescence changes consisted of decreases in the variable to maximum fluorescence ratio and in increases in the relative proportion of variable fluorescence leading to point I in the Kautsky fluorescence induction curve. These relative increases in fluorescence at point I appeared to arise from a delayed plastoquinone reoxidation in the dark, since they disappeared after short, far-red illumination, which is known to excite photosystem I preferentially. We show that a significant correlation existed between some fluorescence parameters, measured after a combined salt and high-light treatment, and other independent measurements of salinity tolerance. These results suggest that chlorophyll fluorescence, and especially the relative fluorescence at point I in the Kautsky fluorescence induction curve, could be used for the screening of barley genotypes for salinity tolerance.
研究了叶绿素荧光测量在筛选耐盐大麦（Hordeum vulgare L.）基因型中的应用。将离体大麦叶片在水下切割，然后将切割端浸入水中或100 mM NaCl溶液中，分别在黑暗或强光条件下进行培养。仅当在强光条件下进行培养时，暴露于盐溶液中的离体大麦叶片才会出现快速荧光动力学变化。荧光变化包括可变荧光与最大荧光比值的降低，以及导致Kautsky荧光诱导曲线中I点的可变荧光相对比例的增加。I点处荧光的这些相对增加似乎是由于黑暗中质体醌再氧化延迟所致，因为在短暂的远红光照射后它们消失了，已知远红光优先激发光系统I。我们表明，在盐和强光联合处理后测量的一些荧光参数与其他耐盐性独立测量之间存在显著相关性。这些结果表明，叶绿素荧光，尤其是Kautsky荧光诱导曲线中I点的相对荧光，可用于筛选耐盐大麦基因型。
