Makhtoum Somayyeh, Sabouri Hossein, Gholizadeh Abdollatif, Ahangar Leila, Katouzi Mahnaz, Mastinu Andrea
Department of Plant Production, Faculty of Agriculture Science and Natural Resources, Gonbad Kavous University, P.O. Box 163, Gonbad 49717-99151, Iran.
Department of Plant Breeding and Genetic Resource, Agroscope, 1260 Nyon, Switzerland.
Plants (Basel). 2023 Oct 9;12(19):3515. doi: 10.3390/plants12193515.
To map the genomic regions and control chlorophyll fluorescence attributes under normal, salinity-, and drought-stress conditions in barley ( L.) at the seedling stage, an experiment was conducted in 2019-2020 using 106 F8 lines resulting from the cross between Badia × Kavir. Initially, the different chlorophyll fluorescence parameters were evaluated. Under drought stress, the highest decrease was related to REo/CSm (59.56%), and the highest increase was related to dV/dto (77.17%). Also, under salinity stress, the highest decrease was related to Fv/Fo (59.56%), and the highest increase was related to DIo/RC (77.17%). Linkage maps were prepared using 152 SSR polymorphic markers, 72 ISSR alleles, 7 IRAP alleles, 29 CAAT alleles, 27 Scot alleles, and 15 iPBS alleles. The obtained map accounted for 999.2 centi-Morgans (cM) of the barley genome length (92% of the whole barley genome). The results indicated the importance of chromosomes 3, 2, and 7 in controlling ABS/CSm, Area, ETo/CSm, Fm, Fv, and ETo/RC under drought stress. qEToRCD-7, as a major QTL, controlled 18.3% of ETo/RC phenotypic variation under drought stress. Under salinity stress, the regions of chromosomes 2 and 7 (102 cM and 126 cM) controlled the parameters ABS/CSo, Fm, Fo, Fv, TRo/SCo, Area, ETo/CSm, and ETo/CSo. The results showed that chlorophyll fluorescence is an important parameter in the study of drought and salinity effects on barley. This is the first report of the investigation of changes in the genetic structure of quantitative genes controlling the fluorescence parameters associated with barley response to drought and salinity stresses in the Iranian barley RILs population. According to the obtained results, it is possible to use HVPLASC1B and EBmac0713 in normal conditions, ISSR21-2 and ISSR30-4 in drought conditions, and Bmac0047, Scot5-B, CAAT6-C, and ISSR30iPBS2076-4 in saline stress conditions to select genotypes with higher photosynthetic capacity in marker-assisted selection programs.
为了绘制大麦（L.）幼苗期在正常、盐胁迫和干旱胁迫条件下的基因组区域并控制叶绿素荧光属性，于2019 - 2020年进行了一项实验，使用了106个由Badia×Kavir杂交产生的F8株系。最初，对不同的叶绿素荧光参数进行了评估。在干旱胁迫下，降幅最大的是REo/CSm（59.56%），增幅最大的是dV/dto（77.17%）。同样，在盐胁迫下，降幅最大的是Fv/Fo（59.56%），增幅最大的是DIo/RC（77.17%）。利用152个SSR多态性标记、72个ISSR等位基因、7个IRAP等位基因、29个CAAT等位基因、27个Scot等位基因和15个iPBS等位基因构建了连锁图谱。所得图谱覆盖大麦基因组长度的999.2厘摩（cM）（占整个大麦基因组的92%）。结果表明，在干旱胁迫下，3号、2号和7号染色体在控制ABS/CSm、Area、ETo/CSm、Fm、Fv和ETo/RC方面具有重要作用。qEToRCD - 7作为一个主要的QTL，在干旱胁迫下控制了ETo/RC表型变异的18.3%。在盐胁迫下，2号和7号染色体区域（102 cM和126 cM）控制了ABS/CSo、Fm、Fo、Fv、TRo/SCo、Area、ETo/CSm和ETo/CSo等参数。结果表明，叶绿素荧光是研究干旱和盐胁迫对大麦影响的一个重要参数。这是关于伊朗大麦重组自交系群体中控制与大麦对干旱和盐胁迫响应相关的荧光参数的数量基因遗传结构变化研究的首次报道。根据所得结果，在正常条件下可以使用HVPLASC1B和EBmac0713，在干旱条件下可以使用ISSR21 - 2和ISSR30 - 4，在盐胁迫条件下可以使用Bmac0047、Scot5 - B、CAAT6 - C和ISSR30iPBS2076 - 4，在标记辅助选择计划中选择具有较高光合能力的基因型。
