大麦（Hordeum vulgare L.）糊粉层组织中的磷酸肌醇
Phosphoinositides in Barley (Hordeum vulgare L.) Aleurone Tissue.
作者信息
Brearley C. A., Hanke D. E.
机构信息
Department of Plant Sciences, Cambridge University, Downing Street, Cambridge CB2 3EA, United Kingdom.
出版信息
Plant Physiol. 1994 Apr;104(4):1381-1384. doi: 10.1104/pp.104.4.1381.
Abstract
[3H]Inositol labeling of barley (Hordeum vulgare L. cv Himalaya) aleurone layers and analysis of phospholipids by deacylation revealed the presence of phosphatidylinositol (PtdIns), PtdIns3P, and PtdIns4P but not PtdInsP2 species. In contrast to an earlier report (P.P.N. Murthy, G. Pliska-Matyshak, L.M. Keranen, P. Lam, H.H. Mueller, N. Bhuvarahamurthy [1992] Plant Physiol 98: 1498-1501) systematic chemical degradation of PtdIns revealed no evidence of a second isomer of PtdIns. Evidence of the widespread occurrence of 3-phosphorylated PtdIns within the plant kingdom is presented.
摘要
用[3H]肌醇标记大麦（Hordeum vulgare L. cv Himalaya）糊粉层，并通过脱酰基作用分析磷脂，结果显示存在磷脂酰肌醇（PtdIns）、磷脂酰肌醇-3-磷酸（PtdIns3P）和磷脂酰肌醇-4-磷酸（PtdIns4P），但不存在磷脂酰肌醇-4,5-二磷酸（PtdInsP2）种类。与早期报告（P.P.N. Murthy、G. Pliska-Matyshak、L.M. Keranen、P. Lam、H.H. Mueller、N. Bhuvarahamurthy [1992] Plant Physiol 98: 1498 - 1501）相反，对PtdIns进行系统的化学降解未发现PtdIns存在第二种异构体的证据。本文提供了植物界中广泛存在3-磷酸化PtdIns的证据。
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