一种用于肌醇磷酸酯及其异构体的快速分离方法。
A rapid separation method for inositol phosphates and their isomers.
作者信息
Wreggett K A, Irvine R F
机构信息
Department of Biochemistry, Agricultural and Food Research Council, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
出版信息
Biochem J. 1987 Aug 1;245(3):655-60. doi: 10.1042/bj2450655.
Abstract
A technique is described using ACCELL QMA anion-exchange SEP-PAKs (Waters Associates) with ammonium formate-based solutions, whereby a sample can be processed within minutes to yield resolution of inositol phosphates. Isomers of inositol trisphosphate can then be separated by using this technique in combination with a rapid (5-6 min) isocratic h.p.l.c. procedure. The use of QMA SEP-PAKs offers a degree of reproducibility comparable with that of h.p.l.c. while maintaining the capacity for automation, allowing large numbers of samples to be processed rapidly.
摘要
本文描述了一种使用ACCELL QMA阴离子交换SEP - PAK柱（沃特世公司）和基于甲酸铵的溶液的技术，通过该技术可在几分钟内处理样品以实现肌醇磷酸的分离。然后，结合快速（5 - 6分钟）等度高效液相色谱法，利用该技术可分离肌醇三磷酸的异构体。使用QMA SEP - PAK柱可提供与高效液相色谱法相当的重现性，同时保持自动化处理能力，从而能够快速处理大量样品。
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