磷酸肌醇异构体的自动等度高效液相色谱法。
Automated isocratic high-performance liquid chromatography of inositol phosphate isomers.
作者信息
Wreggett K A, Irvine R F
机构信息
Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Cambridge, U.K.
出版信息
Biochem J. 1989 Sep 15;262(3):997-1000. doi: 10.1042/bj2620997.
Abstract
Isomers of inositol phosphates from biological samples can be analysed by anion-exchange h.p.l.c., by using isocratic elution with phosphate buffers. The method involves the preliminary processing of the extracted samples with conventional soft-gel anion-exchange resins, including the commonly used Dowex resins, followed by direct analysis with h.p.l.c. of a portion of relevant fractions. Run times (up to 20 min) and collected fraction numbers (up to 24) are minimal, so that if the method is used in conjunction with automated h.p.l.c. injection a high throughput of samples is achieved.
摘要
生物样品中肌醇磷酸酯的异构体可以通过阴离子交换高效液相色谱法进行分析，采用磷酸盐缓冲液等度洗脱。该方法包括用常规软凝胶阴离子交换树脂（包括常用的Dowex树脂）对提取的样品进行预处理，然后对部分相关馏分进行高效液相色谱直接分析。运行时间（最长20分钟）和收集的馏分数量（最多24个）最少，因此如果该方法与自动高效液相色谱进样结合使用，就能实现高通量的样品分析。
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本文引用的文献
1
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J Biol Chem. 1961 Jan;236:54-60.
2
Inositol trisphosphates in carbachol-stimulated rat parotid glands.卡巴胆碱刺激的大鼠腮腺中的肌醇三磷酸
Biochem J. 1984 Oct 1;223(1):237-43. doi: 10.1042/bj2230237.
3
Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides.膜磷酸肌醇在激动剂依赖性水解后肌醇磷酸水平的变化。
Biochem J. 1983 May 15;212(2):473-82. doi: 10.1042/bj2120473.
4
Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices.毒蕈碱受体刺激大鼠大脑皮层切片后肌醇1,3,4,5-四磷酸的快速形成。
Biochem J. 1985 Nov 15;232(1):211-5. doi: 10.1042/bj2320211.
5
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7
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