乙烯诱导巴西橡胶树乳胶细胞中谷氨酰胺合成酶活性及mRNA水平增加。
Ethylene-Induced Increase in Glutamine Synthetase Activity and mRNA Levels in Hevea brasiliensis Latex Cells.
作者信息
Pujade-Renaud V., Clement A., Perrot-Rechenmann C., Prevot J. C., Chrestin H., Jacob J. L., Guern J.
机构信息
Institut des Sciences Vegetales, Centre National de la Recherche Scientifique, bat 22, Avenue de la Terrasse, 91198 Gif-sur-Yvette, Cedex France (V.P.-R., C.P.-R., H.C., J.G.).
出版信息
Plant Physiol. 1994 May;105(1):127-132. doi: 10.1104/pp.105.1.127.
Abstract
Ethylene, used as a stimulant of latex production in Hevea brasiliensis, significantly activates the regenerating metabolism within the laticiferous cells. In this context, attention was focused on glutamine synthetase (GS; EC 6.3.1.2), a key enzyme in nitrogen metabolism. A specific and significant activation of the cytosolic glutamine synthetase (GScyt) in the laticiferous cells after ethylene treatment parallels the increase of latex yield. A marked accumulation of the corresponding mRNA was found, but in contrast, a slight and variable increase of the polypeptide level is at the limit of detection by western blotting. The GS response to ethylene might be mediated by ammonia that increases in latex cytosol following ethylene treatment. The physiological significance for such a regulation by ethylene of the GScyt is discussed in terms of the nitrogen requirement for protein synthesis associated with latex regeneration.
摘要
乙烯被用作巴西橡胶树乳胶生产的刺激剂，它能显著激活乳管细胞内的再生代谢。在此背景下，人们将注意力集中在谷氨酰胺合成酶（GS；EC 6.3.1.2）上，它是氮代谢中的关键酶。乙烯处理后，乳管细胞中的胞质谷氨酰胺合成酶（GScyt）发生特异性且显著的激活，这与乳胶产量的增加同步。研究发现相应的mRNA有明显积累，但相比之下，通过蛋白质印迹法检测到的多肽水平仅略有增加且变化不定。GS对乙烯的反应可能由乙烯处理后乳胶细胞质中增加的氨介导。本文从与乳胶再生相关的蛋白质合成对氮的需求角度讨论了乙烯对GScyt进行这种调节的生理意义。
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