橡胶树无性系 CATAS8 - 79 和 PR107 胶乳的比较转录组分析揭示了胶乳再生调控和胶乳流动持续时间的新线索。
Comparative transcriptome analysis of latex from rubber tree clone CATAS8-79 and PR107 reveals new cues for the regulation of latex regeneration and duration of latex flow.
作者信息
Chao Jinquan, Chen Yueyi, Wu Shaohua, Tian Wei-Min
机构信息
Ministry of Agriculture Key Laboratory of Biology and Genetic Resources of Rubber Tree/ State Key Laboratory Breeding Base of Cultivation and Physiology for Tropical Crops, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, 571737, PR China.
出版信息
BMC Plant Biol. 2015 Apr 18;15:104. doi: 10.1186/s12870-015-0488-3.
BACKGROUND
Rubber tree (Hevea brasiliensis Muell. Arg.) is the primarily commercial source of natural rubber in the world. Latex regeneration and duration of latex flow after tapping are the two factors that determine rubber yield of rubber tree, and exhibit a huge variation between rubber tree clones CATAS8-79 and PR107.
RESULTS
To dissect the molecular mechanism for the regulation of latex regeneration and duration of latex flow, we sequenced and comparatively analyzed latex of rubber tree clone CATAS8-79 and PR107 at transriptome level. More than 26 million clean reads were generated in each pool and 51,829 all-unigenes were totally assembled. A total of 6,726 unigenes with differential expression patterns were detected between CATAS8-79 and PR107. Functional analysis showed that genes related to mass of categories were differentially enriched between the two clones. Expression pattern of genes which were involved in latex regeneration and duration of latex flow upon successive tapping was analyzed by quantitative PCR. Several genes related to rubber biosynthesis, cellulose and lignin biosynthesis and rubber particle aggregation were differentially expressed between CATAS8-79 and PR107.
CONCLUSIONS
This is the first report about probing latex regeneration and duration of latex flow by comparative transcriptome analysis. Among all the suggested factors, it is more important that the level of endogenous jasmonates, carbohydrate metabolism, hydroxymethylglutaryl-CoA reductase (HMGR) and Hevea rubber transferase (HRT) in mevalonate (MVA) parthway for latex regeneration while the level of endogenous ethylene (ETH), lignin content of laticifer cell wall, antioxidants and glucanases for the duration of latex flow. These data will provide new cues for understanding the molecular mechanism for the regulation of latex regeneration and duration of latex flow in rubber tree.
背景
橡胶树（巴西橡胶树穆勒·阿古）是世界上天然橡胶的主要商业来源。割胶后乳胶再生和乳胶流动持续时间是决定橡胶树橡胶产量的两个因素，并且在橡胶树品种热研8-79和PR107之间表现出巨大差异。
结果
为了解析调控乳胶再生和乳胶流动持续时间的分子机制，我们在转录组水平对橡胶树品种热研8-79和PR107的乳胶进行了测序和比较分析。每个样本库产生了超过2600万个clean reads，共组装得到51829个单基因。在热研8-79和PR107之间共检测到6726个具有差异表达模式的单基因。功能分析表明，两个品种之间与多个类别的基因差异富集。通过定量PCR分析了连续割胶后参与乳胶再生和乳胶流动持续时间的基因的表达模式。热研8-79和PR107之间，几个与橡胶生物合成、纤维素和木质素生物合成以及橡胶粒子聚集相关的基因差异表达。
结论
这是首次通过比较转录组分析探究乳胶再生和乳胶流动持续时间的报告。在所有提出的因素中，对于乳胶再生而言，内源性茉莉酸水平、碳水化合物代谢、甲羟戊酸（MVA）途径中的3-羟基-3-甲基戊二酰辅酶A还原酶（HMGR）和橡胶树橡胶转移酶（HRT）更为重要；而对于乳胶流动持续时间来说，内源性乙烯（ETH）水平、乳管细胞壁木质素含量、抗氧化剂和葡聚糖酶更为重要。这些数据将为理解橡胶树乳胶再生和乳胶流动持续时间调控的分子机制提供新线索。
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