College of Life Sciences, Key Laboratory of Xinjiang Phytomedicine Resource Utilization of Ministry of Education, Shihezi University, Shihezi 832003, China.
College of Life Sciences, Ministry of Education Key Laboratory for Ecology of Tropical Islands, Hainan Normal University, Haikou 571158, China.
Int J Mol Sci. 2018 Mar 22;19(4):947. doi: 10.3390/ijms19040947.
Natural rubber latex production can be improved by ethylene stimulation in the rubber tree (). However, the expression levels of most functional proteins for natural rubber biosynthesis are not induced after ethylene application, indicating that post-translational modifications, especially protein phosphorylation, may play important roles in ethylene signaling in . Here, we performed a comprehensive investigation on evolution, ethylene-induced expression and protein-protein interaction of calcium-dependent protein kinases (CPKs), an important serine/threonine protein kinase family, in . Nine duplication events were determined in the 30 identified genes. Expression profiling of in three rubber tree cultivars with low, medium and high ethylene sensitivity showed that , , , , , and are induced by ethylene in at least one cultivar. Evolution rate analysis suggested accelerated evolution rates in two paralogue pairs, and Analysis of proteomic data for rubber latex after ethylene treatment showed that seven HbCPK proteins could be detected, including six ethylene-induced ones. Protein-protein interaction analysis of the 493 different abundant proteins revealed that protein kinases, especially calcium-dependent protein kinases, possess most key nodes of the interaction network, indicating that protein kinase and protein phosphorylation play important roles in ethylene signaling in latex of . In summary, our data revealed the expression patterns of family members and functional divergence of two paralogue pairs, as well as the potential important roles of in ethylene-induced rubber production improvement in .
天然橡胶乳胶的生产可以通过橡胶树中的乙烯刺激来提高（）。然而，大多数天然橡胶生物合成的功能蛋白的表达水平在乙烯应用后并没有被诱导，这表明翻译后修饰，特别是蛋白质磷酸化，可能在 中乙烯信号转导中发挥重要作用。在这里，我们对钙依赖性蛋白激酶（CPKs）这一重要的丝氨酸/苏氨酸蛋白激酶家族在中的进化、乙烯诱导表达和蛋白-蛋白相互作用进行了全面研究。在 30 个鉴定的基因中确定了 9 个重复事件。对 3 个具有低、中、高乙烯敏感性的橡胶树品种中的 表达谱进行了研究，结果表明，至少在一个品种中， 、 、 、 、 和 被乙烯诱导。进化率分析表明，在两个同源基因对 和 中存在加速进化的趋势。对乙烯处理后的橡胶乳液进行蛋白质组学数据分析表明，有 7 个 HbCPK 蛋白可以被检测到，其中 6 个是乙烯诱导的。对 493 种不同丰度蛋白质的蛋白-蛋白相互作用分析表明，蛋白激酶，特别是钙依赖性蛋白激酶，拥有相互作用网络的大多数关键节点，这表明蛋白激酶和蛋白磷酸化在乙烯诱导的乳胶中乙烯信号转导中发挥重要作用。综上所述，我们的数据揭示了 家族成员的表达模式和两个 同源基因对的功能分化，以及 在乙烯诱导提高橡胶产量中的潜在重要作用。
