两种木本植物叶片在光照下估算暗呼吸方法的比较
Comparison of Methods to Estimate Dark Respiration in the Light in Leaves of Two Woody Species.
作者信息
Villar R., Held A. A., Merino J.
机构信息
Departamento de Biologia Vegetal y Ecologia, Universidad de Sevilla, Apdo 1095, 41080 Sevilla, Spain (R.V., J.M.).
出版信息
Plant Physiol. 1994 May;105(1):167-172. doi: 10.1104/pp.105.1.167.
Dark respiration in the light was estimated in leaves of two woody species (Heteromeles arbutifolia Ait. and Lepechinia fragans Greene) using two different approaches based on gas-exchange techniques: the Kok method and the Laisk method. In all cases, dark respiration in the light was lower (P < 0.05) than respiration in darkness, indicating that dark respiration was inhibited in the light. Rates of dark respiration in the light estimated by the Laisk method were 52% higher (P < 0.05) than those estimated by the Kok method. Differences between the methods could be explained by the low ambient CO2 concentrations required by the Laisk approach. The mean value of the inhibition of respiration by light for the two species, corrected for the ambient CO2 concentration effect, was 55%. Despite the differences in leaf characteristics between the species, values of the CO2 photocompensation point, at which the rate of photosynthetic CO2 uptake equaled that of photorespiratory CO2 evolution, were very constant, suggesting an excellent consistency in the results obtained with the Laisk approach.
利用基于气体交换技术的两种不同方法（Kok法和Laisk法），对两种木本植物（草莓树和芳香石蚕叶香科科）叶片在光照下的暗呼吸进行了估算。在所有情况下，光照下的暗呼吸均低于黑暗中的呼吸（P < 0.05），这表明光照会抑制暗呼吸。Laisk法估算的光照下暗呼吸速率比Kok法估算的高52%（P < 0.05）。两种方法之间的差异可以通过Laisk法所需的低环境CO₂浓度来解释。校正环境CO₂浓度效应后，两种植物光照对呼吸抑制的平均值为55%。尽管两种植物的叶片特征存在差异，但光合CO₂吸收速率等于光呼吸CO₂释放速率时的CO₂光补偿点的值非常恒定，这表明Laisk法获得的结果具有良好的一致性。
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