异源三聚体G蛋白α亚基在水稻抗病性中作用于小GTP酶Rac的上游。
The heterotrimeric G protein alpha subunit acts upstream of the small GTPase Rac in disease resistance of rice.
作者信息
Suharsono Utut, Fujisawa Yukiko, Kawasaki Tsutomu, Iwasaki Yukimoto, Satoh Hikaru, Shimamoto Ko
机构信息
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.
出版信息
Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13307-12. doi: 10.1073/pnas.192244099. Epub 2002 Sep 17.
Abstract
We used rice dwarf1 (d1) mutants lacking a single-copy Galpha gene and addressed Galpha's role in disease resistance. d1 mutants exhibited a highly reduced hypersensitive response to infection by an avirulent race of rice blast. Activation of PR gene expression in the leaves of the mutants infected with rice blast was delayed for 24 h relative to the wild type. H(2)O(2) production and PR gene expression induced by sphingolipid elicitors (SE) were strongly suppressed in d1 cell cultures. Expression of the constitutively active OsRac1, a small GTPase Rac of rice, in d1 mutants restored SE-dependent defense signaling and resistance to rice blast. Galpha mRNA was induced by an avirulent race of rice blast and SE application on the leaf. These results indicated the role of Galpha in R gene-mediated disease resistance of rice. We have proposed a model for the defense signaling of rice in which the heterotrimeric G protein functions upstream of the small GTPase OsRac1 in the early steps of signaling.
摘要
我们使用了缺失单拷贝Gα基因的水稻矮化1（d1）突变体，并研究了Gα在抗病性中的作用。d1突变体对无毒稻瘟病菌株感染的过敏反应显著降低。相对于野生型，感染稻瘟病的突变体叶片中PR基因表达的激活延迟了24小时。在d1细胞培养物中，鞘脂激发子（SE）诱导的H₂O₂产生和PR基因表达受到强烈抑制。组成型活性水稻小GTP酶Rac（OsRac1）在d1突变体中的表达恢复了SE依赖性防御信号传导和对稻瘟病的抗性。无毒稻瘟病菌株和在叶片上施用SE可诱导Gα mRNA表达。这些结果表明了Gα在水稻R基因介导的抗病性中的作用。我们提出了一个水稻防御信号传导模型，其中异源三聚体G蛋白在信号传导的早期步骤中在小GTP酶OsRac1上游起作用。
相似文献
1
The heterotrimeric G protein alpha subunit acts upstream of the small GTPase Rac in disease resistance of rice.异源三聚体G蛋白α亚基在水稻抗病性中作用于小GTP酶Rac的上游。
Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13307-12. doi: 10.1073/pnas.192244099. Epub 2002 Sep 17.
2
Essential role of the small GTPase Rac in disease resistance of rice.小GTP酶Rac在水稻抗病性中的重要作用
Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):759-64. doi: 10.1073/pnas.98.2.759. Epub 2001 Jan 9.
3
The crystal structure of the plant small GTPase OsRac1 reveals its mode of binding to NADPH oxidase.植物小GTP酶OsRac1的晶体结构揭示了其与NADPH氧化酶的结合模式。
J Biol Chem. 2014 Oct 10;289(41):28569-78. doi: 10.1074/jbc.M114.603282. Epub 2014 Aug 15.
4
Proteomics of Rac GTPase signaling reveals its predominant role in elicitor-induced defense response of cultured rice cells.Rac GTPase信号传导的蛋白质组学揭示了其在诱导培养水稻细胞防御反应中的主要作用。
Plant Physiol. 2006 Feb;140(2):734-45. doi: 10.1104/pp.105.068395. Epub 2005 Dec 29.
5
Heterotrimeric G protein signaling is required for epidermal cell death in rice.异源三聚体G蛋白信号传导是水稻表皮细胞死亡所必需的。
Plant Physiol. 2009 Oct;151(2):732-40. doi: 10.1104/pp.109.142133. Epub 2009 Aug 5.
6
A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice 1[w].一种鞘脂激发子诱导的丝裂原活化蛋白激酶受水稻中小GTP酶OsRac1和异源三聚体G蛋白的调控1[w] 。
Plant Physiol. 2005 Jul;138(3):1644-52. doi: 10.1104/pp.104.057414. Epub 2005 Jun 10.
7
Rice dwarf mutant d1, which is defective in the alpha subunit of the heterotrimeric G protein, affects gibberellin signal transduction.水稻矮化突变体d1在异源三聚体G蛋白的α亚基上存在缺陷，影响赤霉素信号转导。
Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11638-43. doi: 10.1073/pnas.97.21.11638.
8
Importance of OsRac1 and RAI1 in signalling of nucleotide-binding site leucine-rich repeat protein-mediated resistance to rice blast disease.OsRac1 和 RAI1 在核苷酸结合位点富含亮氨酸重复蛋白介导的稻瘟病抗性信号转导中的重要性。
New Phytol. 2019 Jul;223(2):828-838. doi: 10.1111/nph.15816. Epub 2019 Apr 19.
9
Palmitoylation-dependent membrane localization of the rice resistance protein pit is critical for the activation of the small GTPase OsRac1.水稻抗病蛋白Pit的棕榈酰化依赖性膜定位对于小GTP酶OsRac1的激活至关重要。
J Biol Chem. 2014 Jul 4;289(27):19079-88. doi: 10.1074/jbc.M114.569756. Epub 2014 May 19.
10
Proteomic analysis of rice embryo: an approach for investigating Galpha protein-regulated proteins.水稻胚的蛋白质组学分析：一种研究Gα蛋白调控蛋白的方法。
Proteomics. 2005 Oct;5(15):3932-41. doi: 10.1002/pmic.200401237.
引用本文的文献
1
CO signalling in plants.植物中的一氧化碳信号传导
Philos Trans R Soc Lond B Biol Sci. 2025 May 29;380(1927):20240247. doi: 10.1098/rstb.2024.0247.
2
The role of OsRGA1 in aerenchyma formation and adventitious root growth in rice seedlings based on the U-Gompertz model.基于U-冈珀茨模型研究OsRGA1在水稻幼苗通气组织形成和不定根生长中的作用。
BMC Plant Biol. 2025 Apr 17;25(1):490. doi: 10.1186/s12870-025-06526-6.
3
RGA1 regulates grain size, rice quality and seed germination in the small and round grain mutant srg5.RGA1 调控小粒圆粒突变体 srg5 的粒型、稻米品质和种子萌发。
BMC Plant Biol. 2024 Mar 4;24(1):167. doi: 10.1186/s12870-024-04864-5.
4
The hybrid lethality of interspecific F hybrids of : a clue to understanding hybrid inviability-a major obstacle to wide hybridization and introgression breeding of plants.种间F杂种的杂种致死性：理解杂种不育性的线索——植物远缘杂交和渐渗育种的主要障碍
Mol Breed. 2022 Feb 11;42(2):10. doi: 10.1007/s11032-022-01279-8. eCollection 2022 Feb.
5
Extra-large G proteins have extra-large effects on agronomic traits and stress tolerance in maize and rice.超大 G 蛋白对玉米和水稻的农艺性状和抗逆性有显著影响。
Trends Plant Sci. 2023 Sep;28(9):1033-1044. doi: 10.1016/j.tplants.2023.04.005. Epub 2023 May 7.
6
Novel Insights into Understanding the Molecular Dialogues between Bipolaroxin and the Gα and Gβ Subunits of the Wheat Heterotrimeric G-Protein during Host-Pathogen Interaction.关于理解双极毒素与小麦异源三聚体G蛋白的Gα和Gβ亚基在宿主-病原体相互作用过程中分子对话的新见解。
Antioxidants (Basel). 2022 Sep 5;11(9):1754. doi: 10.3390/antiox11091754.
7
Polyamine: A Potent Ameliorator for Plant Growth Response and Adaption to Abiotic Stresses Particularly the Ammonium Stress Antagonized by Urea.多胺：一种促进植物生长反应和适应非生物胁迫（尤其是尿素拮抗铵胁迫）的有效改良剂。
Front Plant Sci. 2022 Mar 23;13:783597. doi: 10.3389/fpls.2022.783597. eCollection 2022.
8
The multifaceted roles of heterotrimeric G-proteins: lessons from models and crops.三聚体 G 蛋白的多面角色：来自模型和作物的启示。
Planta. 2022 Mar 19;255(4):88. doi: 10.1007/s00425-022-03868-5.
9
A small Rho GTPase OsRacB is required for pollen germination in rice.一个小的 Rho GTPase OsRacB 是水稻花粉萌发所必需的。
Dev Growth Differ. 2022 Feb;64(2):88-97. doi: 10.1111/dgd.12752. Epub 2021 Oct 5.
10
Heterotrimeric G protein γ subunit DEP1 is involved in hydrogen peroxide signaling and promotes aerenchyma formation in rice roots.三聚体 G 蛋白 γ 亚基 DEP1 参与过氧化氢信号转导，并促进水稻根通气组织的形成。
Plant Signal Behav. 2021 May 4;16(5):1889251. doi: 10.1080/15592324.2021.1889251. Epub 2021 Feb 26.
本文引用的文献
1
Light signal transduction in plants.植物中的光信号转导。
Trends Cell Biol. 1997 Jan;7(1):21-6. doi: 10.1016/S0962-8924(97)10043-5.
2
Plant Defense Response to Fungal Pathogens (II. G-Protein-Mediated Changes in Host Plasma Membrane Redox Reactions).植物对真菌病原体的防御反应（II. G蛋白介导的宿主质膜氧化还原反应变化）
Plant Physiol. 1994 Sep;106(1):97-102. doi: 10.1104/pp.106.1.97.
3
Activation of Plant Plasma Membrane Ca2+-Permeable Channels by Race-Specific Fungal Elicitors.种特异性真菌激发子对植物质膜钙离子通透通道的激活作用
Plant Physiol. 1997 Jan;113(1):269-279. doi: 10.1104/pp.113.1.269.
4
A mutant Arabidopsis heterotrimeric G-protein beta subunit affects leaf, flower, and fruit development.一种突变的拟南芥异源三聚体G蛋白β亚基影响叶片、花朵和果实的发育。
Plant Cell. 2001 Dec;13(12):2631-41. doi: 10.1105/tpc.010315.
5
Structure and function of heterotrimeric G proteins in plants.植物中异源三聚体G蛋白的结构与功能
Plant Cell Physiol. 2001 Aug;42(8):789-94. doi: 10.1093/pcp/pce111.
6
Superoxide production by plant homologues of the gp91(phox) NADPH oxidase. Modulation of activity by calcium and by tobacco mosaic virus infection.gp91(phox) 烟酰胺腺嘌呤二核苷酸磷酸氧化酶植物同源物产生超氧化物。钙和烟草花叶病毒感染对活性的调节。
Plant Physiol. 2001 Jul;126(3):1281-90. doi: 10.1104/pp.126.3.1281.
7
Overexpression of the heterotrimeric G-protein alpha-subunit enhances phytochrome-mediated inhibition of hypocotyl elongation in Arabidopsis.异源三聚体G蛋白α亚基的过表达增强了拟南芥中光敏色素介导的下胚轴伸长抑制作用。
Plant Cell. 2001 Jul;13(7):1639-52. doi: 10.1105/tpc.010008.
8
G protein regulation of ion channels and abscisic acid signaling in Arabidopsis guard cells.拟南芥保卫细胞中G蛋白对离子通道及脱落酸信号的调控
Science. 2001 Jun 15;292(5524):2070-2. doi: 10.1126/science.1059046.
9
Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis.拟南芥中异源三聚体G蛋白对细胞增殖的调控
Science. 2001 Jun 15;292(5524):2066-9. doi: 10.1126/science.1059040.
10
Essential role of the small GTPase Rac in disease resistance of rice.小GTP酶Rac在水稻抗病性中的重要作用
Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):759-64. doi: 10.1073/pnas.98.2.759. Epub 2001 Jan 9.
Zotero 插件