Wild Margaret A, Spraker Terry R, Sigurdson Christina J, O'Rourke Katherine I, Miller Michael W
Colorado Division of Wildlife, Wildlife Research Center, 317 W. Prospect Road, Fort Collins, CO 80526, USA1.
Colorado State Veterinary Diagnostic Laboratory, Colorado State University, Fort Collins, CO 80523, USA2.
J Gen Virol. 2002 Oct;83(Pt 10):2629-2634. doi: 10.1099/0022-1317-83-10-2629.
The usefulness of tonsillar biopsy on live deer for preclinical diagnosis of the transmissible spongiform encephalopathy chronic wasting disease (CWD) was evaluated. Disease was tracked in a CWD-endemic herd using serial tonsillar biopsies collected at 6 to 9 month intervals from 34 captive mule deer (Odocoileus hemionus) and five white-tailed deer (O. virginianus). Tonsillar biopsies were examined for accumulation of PrP(CWD), the protein marker for infection, using immunohistochemical (IHC) staining. 26/34 (76%) mule deer and 4/5 (80%) white-tailed deer had PrP(CWD) accumulation in tonsillar biopsies; CWD was subsequently confirmed by post-mortem examination in all 30 of these tonsillar-positive deer. Six mule deer with IHC-negative tonsillar biopsies had positive brain and tonsillar IHC staining upon death 12 to 40 months following the last biopsy. PrP(CWD) accumulation in tonsillar biopsy was observed 2 to 20 months before CWD-related death and up to 14 months before onset of clinical signs of CWD. Tonsillar biopsies from 3-month-old mule deer (n=6) were IHC negative, but PrP(CWD) accumulation was detected in tonsillar biopsies from 7/10 mule deer by 19 months of age. Tonsillar biopsy evaluated with IHC staining is a useful technique for the preclinical diagnosis of CWD in live mule deer and white-tailed deer when intensive management approaches are possible.
评估了对活体鹿进行扁桃体活检用于慢性消耗病（CWD）这一传染性海绵状脑病临床前诊断的效用。在一个CWD地方病流行的鹿群中，对34只圈养骡鹿（白尾鹿属）和5只白尾鹿每隔6至9个月采集系列扁桃体活检样本，追踪疾病情况。使用免疫组织化学（IHC）染色检查扁桃体活检样本中感染的蛋白质标志物PrP（CWD）的积累情况。26/34（76%）的骡鹿和4/5（80%）的白尾鹿扁桃体活检样本中有PrP（CWD）积累；随后在所有这30只扁桃体阳性的鹿中通过尸检确认了CWD。6只扁桃体活检IHC阴性的骡鹿在最后一次活检后12至40个月死亡时，脑和扁桃体的IHC染色呈阳性。在与CWD相关的死亡前2至20个月以及CWD临床症状出现前长达14个月，观察到扁桃体活检中有PrP（CWD）积累。3个月大的骡鹿（n = 6）的扁桃体活检IHC呈阴性，但到19个月大时，在7/10的骡鹿扁桃体活检中检测到PrP（CWD）积累。当可以采用强化管理方法时，用IHC染色评估的扁桃体活检是对活体骡鹿和白尾鹿进行CWD临床前诊断的有用技术。
