Picasso-Risso Catalina, Schwabenlander Marc D, Rowden Gage, Carstensen Michelle, Bartz Jason C, Larsen Peter A, Wolf Tiffany M
Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN 55108, USA.
Minnesota Center for Prion Research and Outreach, University of Minnesota, Saint Paul, MN 55108, USA.
Pathogens. 2022 Apr 20;11(5):489. doi: 10.3390/pathogens11050489.
Chronic wasting disease (CWD) is a transmissible prion disease of the family. ELISA and IHC tests performed postmortem on the medial retropharyngeal lymph nodes (RPLN) or obex are considered diagnostic gold standards for prion detection. However, differences in CWD transmission, stage of infection, pathogenesis, and strain can limit performance. To overcome these uncertainties, we used Bayesian statistics to assess the accuracy of RT-QuIC, an increasingly used prion amplification assay, to diagnose CWD on tonsil (TLN), parotid (PLN) and submandibular lymph nodes (SMLN), and ELISA/IHC on RPLN of white-tailed deer (WTD) sampled from Minnesota. Dichotomous RT-QuIC and ELISA/IHC results from wild ( = 61) and captive ( = 46) WTD were analyzed with two-dependent-test, one-population models. RT-QuIC performed on TLN and SMLN of the wild WTD population had similar sensitivity (median range (MR): 92.2-95.1) to ELISA/IHC on RPLN (MR: 91.1-92.3). Slightly lower (4-7%) sensitivity estimates were obtained from farmed animal and PLN models. RT-QuIC specificity estimates were high (MR: 94.5-98.5%) and similar to ELISA/IHC estimates (MR: 95.7-97.6%) in all models. This study offers new insights on RT-QuIC and ELISA/IHC performance at the population level and under field conditions, an important step in CWD diagnosis and management.
慢性消耗病（CWD）是该家族的一种可传播的朊病毒病。对咽后内侧淋巴结（RPLN）或延髓后区进行死后ELISA和免疫组化检测被认为是朊病毒检测的诊断金标准。然而，CWD传播、感染阶段、发病机制和毒株的差异可能会限制检测性能。为了克服这些不确定性，我们使用贝叶斯统计来评估RT-QuIC（一种越来越常用的朊病毒扩增检测方法）在诊断来自明尼苏达州的白尾鹿（WTD）扁桃体（TLN）、腮腺（PLN）和下颌下淋巴结（SMLN）中的CWD以及RPLN上的ELISA/免疫组化检测的准确性。采用双依赖检验单总体模型分析了野生（n = 61）和圈养（n = 46）WTD的二分法RT-QuIC和ELISA/免疫组化结果。对野生WTD群体的TLN和SMLN进行的RT-QuIC检测与RPLN上的ELISA/免疫组化检测具有相似的敏感性（中位数范围（MR）：92.2 - 95.1）（MR：91.1 - 92.3）。从养殖动物和PLN模型获得的敏感性估计值略低（4 - 7%）。在所有模型中，RT-QuIC的特异性估计值都很高（MR：94.5 - 98.5%），且与ELISA/免疫组化估计值相似（MR：95.7 - 97.6%）。本研究为RT-QuIC和ELISA/免疫组化在群体水平和野外条件下的性能提供了新的见解，这是CWD诊断和管理中的重要一步。
